Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, 570228 Haikou, China.
Int J Biol Macromol. 2023 Jun 30;241:124570. doi: 10.1016/j.ijbiomac.2023.124570. Epub 2023 Apr 25.
Areca catechu is well known as a medicinal plant that has high nutritional and medicinal benefits. However, the metabolism and regulatory mechanism of B vitamins during areca nut development remain largely unclear. In this study, we obtained the metabolite profiles of six B vitamins during different areca nut developmental stages by targeted metabolomics. Furthermore, we obtained a panoramic expression profile of genes related to the biosynthetic pathway of B vitamins in areca nuts at different developmental stages using RNA-seq. In total, 88 structural genes related to B vitamin biosynthesis were identified. Furthermore, the integrated analysis of B vitamin metabolism data and RNA-seq data showed the key transcription factors regulating thiamine and riboflavin accumulation in areca nuts, including AcbZIP21, AcMYB84, and AcARF32. These results lay the foundation for understanding metabolite accumulation and the molecular regulatory mechanisms of B vitamins in A. catechu nut.
槟榔是一种众所周知的药用植物，具有很高的营养价值和药用价值。然而，槟榔发育过程中 B 族维生素的代谢和调节机制在很大程度上尚不清楚。在这项研究中，我们通过靶向代谢组学获得了六种 B 族维生素在不同槟榔发育阶段的代谢物谱。此外，我们还通过 RNA-seq 获得了不同发育阶段槟榔中与 B 族维生素生物合成途径相关基因的全景表达谱。总共鉴定出 88 个与 B 族维生素生物合成相关的结构基因。此外，B 族维生素代谢数据和 RNA-seq 数据的综合分析表明，调控槟榔中硫胺素和核黄素积累的关键转录因子包括 AcbZIP21、AcMYB84 和 AcARF32。这些结果为理解 A. catechu 坚果中代谢物积累和 B 族维生素的分子调节机制奠定了基础。
