Lai Jun, Li Chun, Zhang Yueran, Wu Zeyong, Li Weiguan, Zhang Zhonghui, Ye Weizhen, Guo Hao, Wang Chao, Long Tuan, Wang Shouchuang, Yang Jun
Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China.
College of Tropical Crops, Hainan University, Haikou 572208, China.
J Agric Food Chem. 2023 Mar 29;71(12):4851-4862. doi: 10.1021/acs.jafc.2c08864. Epub 2023 Mar 20.
L., of the Arecaceae family, is widely distributed in tropical Asia. In , the extracts and compounds, including flavonoids, have various pharmacological activities. Although there are many studies of flavonoids, the molecular mechanism of their biosynthesis and regulation remains unclear in . In this study, 331 metabolites were identified from the root, stem, and leaf of using untargeted metabolomics, including 107 flavonoids, 71 lipids, 44 amino acids and derivatives, and 33 alkaloids. The transcriptome analysis identified 6119 differentially expressed genes, and some were enriched in the flavonoid pathway. To analyze the biosynthetic mechanism of the metabolic differences in tissues, 36 genes were identified through combined transcriptomic and metabolomic analysis, in which glycosyltransferase genes and were annotated as being involved in the glycosylation of kaempferol and chrysin by their expression and in vitro activities. Flavonoid biosynthesis could be regulated by the transcription factors, and . This study laid a foundation for further research on the flavonoid biosynthetic pathway of .
棕榈科的L.在热带亚洲广泛分布。在……中，包括黄酮类化合物在内的提取物和化合物具有多种药理活性。尽管对黄酮类化合物有很多研究，但在……中其生物合成和调控的分子机制仍不清楚。在本研究中，通过非靶向代谢组学从……的根、茎和叶中鉴定出331种代谢物，包括107种黄酮类化合物、71种脂质、44种氨基酸及其衍生物和33种生物碱。转录组分析鉴定出6119个差异表达基因，其中一些在黄酮类途径中富集。为了分析……组织中代谢差异的生物合成机制，通过转录组和代谢组联合分析鉴定出36个基因，其中糖基转移酶基因……通过其表达和体外活性被注释为参与山奈酚和白杨素的糖基化。黄酮类生物合成可能受转录因子……调控。本研究为进一步研究……的黄酮类生物合成途径奠定了基础。
