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通过 RAW264.7 细胞中巨噬细胞的激活和自噬研究叶的免疫刺激活性。

Immunostimulatory Activity of Leaves through Macrophage Activation and Macrophage Autophagy in Mouse Macrophages, RAW264.7 Cells.

机构信息

Department of Forest Science, Andong National University, Andong 36729, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2023 Jul 28;33(7):934-940. doi: 10.4014/jmb.2301.01039. Epub 2023 Apr 19.

DOI:10.4014/jmb.2301.01039
PMID:37100760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10394334/
Abstract

(SP) is a traditional medicinal plant. SP has been reported to have anti-inflammatory, anticancer, and anti-human immunodeficiency virus (HIV) activities. However, there is currently no research available on the immunostimulatory activity of SP. Therefore, in this study, we report that leaves (SPL) activate macrophages. Increased secretion of both immunostimulatory mediators and phagocytic activity was observed in SPL-treated RAW264.7 cells. However, this effect was reversed by the inhibition of TLR2/4. In addition, inhibition of p38 decreased the secretion of immunostimulatory mediators induced by SPL, and inhibition of TLR2/4 decreased the phosphorylation of p38 induced by SPL. SPL augmented p62/SQSTM1 and LC3-II expression. The increase in protein levels of p62/SQSTM1 and LC3-II induced by SPL was decreased by the inhibition of TLR2/4. The results obtained from this study suggest that SPL activates macrophages via TLR2/4-dependent p38 activation and induces autophagy in macrophages via TLR2/4 stimulation.

摘要

（SP）是一种传统药用植物。已有报道称，SP 具有抗炎、抗癌和抗人类免疫缺陷病毒（HIV）的活性。然而，目前尚无关于 SP 的免疫刺激活性的研究。因此，在本研究中，我们报告称 叶（SPL）可激活巨噬细胞。在 SPL 处理的 RAW264.7 细胞中，观察到免疫刺激介质和吞噬活性的分泌均增加。然而，这种作用被 TLR2/4 的抑制所逆转。此外，p38 的抑制减少了 SPL 诱导的免疫刺激介质的分泌，而 TLR2/4 的抑制减少了 SPL 诱导的 p38 的磷酸化。SPL 增加了 p62/SQSTM1 和 LC3-II 的表达。SPL 诱导的 p62/SQSTM1 和 LC3-II 蛋白水平的增加被 TLR2/4 的抑制所降低。本研究的结果表明，SPL 通过 TLR2/4 依赖性 p38 激活来激活巨噬细胞，并通过 TLR2/4 刺激诱导巨噬细胞自噬。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/33dbc78db69a/jmb-33-7-934-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/5db812fe43da/jmb-33-7-934-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/436ba08556a2/jmb-33-7-934-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/3efb65ff590a/jmb-33-7-934-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/9708a12e50ce/jmb-33-7-934-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/33dbc78db69a/jmb-33-7-934-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/5db812fe43da/jmb-33-7-934-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/436ba08556a2/jmb-33-7-934-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/3efb65ff590a/jmb-33-7-934-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/9708a12e50ce/jmb-33-7-934-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/10394334/33dbc78db69a/jmb-33-7-934-f5.jpg

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本文引用的文献

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Pharmazie. 2022 May 1;77(5):147-151. doi: 10.1691/ph.2022.2321.
2
Immune response in COVID-19: what is next?COVID-19 中的免疫反应：下一步是什么？
Cell Death Differ. 2022 Jun;29(6):1107-1122. doi: 10.1038/s41418-022-01015-x. Epub 2022 May 17.
3
Food nutrients as inherent sources of immunomodulation during COVID-19 pandemic.在新冠疫情期间，食物营养素作为免疫调节的内在来源。
Lebensm Wiss Technol. 2022 Mar 15;158:113154. doi: 10.1016/j.lwt.2022.113154. Epub 2022 Jan 31.
4
SARS-CoV-2, COVID-19 and the Ageing Immune System.SARS-CoV-2、COVID-19 和衰老的免疫系统。
Nat Aging. 2021 Sep;1(9):769-782. doi: 10.1038/s43587-021-00114-7. Epub 2021 Sep 14.
5
Structural features and immunomodulatory activities of polysaccharides of longan pulp.龙眼果肉多糖的结构特征与免疫调节活性
Carbohydr Polym. 2012 Jan 4;87(1):636-643. doi: 10.1016/j.carbpol.2011.08.034. Epub 2011 Aug 22.
6
Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway.蝉拟青霉中提取的一种纯化多糖通过激活 TLR4-MAPK-NF-κB 信号通路对 RAW264.7 细胞的免疫调节作用机制。
Int J Biol Macromol. 2020 Dec 1;164:4329-4338. doi: 10.1016/j.ijbiomac.2020.09.035. Epub 2020 Sep 11.
7
Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy.通过促进自噬作用，TLR4 激活的合成糖脂佐剂的疫苗佐剂活性。
Sci Rep. 2020 May 21;10(1):8422. doi: 10.1038/s41598-020-65422-1.
8
Autophagy and Macrophage Functions: Inflammatory Response and Phagocytosis.自噬与巨噬细胞功能：炎症反应与吞噬作用。
Cells. 2019 Dec 27;9(1):70. doi: 10.3390/cells9010070.
9
The Phagocytic Function of Macrophage-Enforcing Innate Immunity and Tissue Homeostasis.巨噬细胞的吞噬功能——先天免疫与组织动态平衡的执行者。
Int J Mol Sci. 2017 Dec 29;19(1):92. doi: 10.3390/ijms19010092.
10
Monitoring and Measuring Autophagy.监测与测量自噬
Int J Mol Sci. 2017 Aug 28;18(9):1865. doi: 10.3390/ijms18091865.