Mitxelena-Iribarren Oihane, Bujanda Xabier, Zabalza Laura, Alkorta Janire, Lopez-Elorza Aitziber, Gracia Raquel, Dupin Damien, Arana Sergio, Ruiz-Cabello Jesús, Mujika Maite
CEIT-Basque Research and Technology Alliance (BRTA), Donostia-San Sebastián, Spain.
Universidad de Navarra, Tecnun, Donostia-San Sebastián, Spain.
Biotechnol J. 2023 Jul;18(7):e2300004. doi: 10.1002/biot.202300004. Epub 2023 Apr 26.
The development of functional blood vessels is today a fundamental pillar in the evaluation of new therapies and diagnostic agents. This article describes the manufacture and subsequent functionalization, by means of cell culture, of a microfluidic device with a circular section. Its purpose is to simulate a blood vessel in order to test new treatments for pulmonary arterial hypertension. The manufacture was carried out using a process in which a wire with a circular section determines the dimensions of the channel. To fabricate the blood vessel, cells were seeded under rotary cell culture to obtain a homogeneous cell seeding in the inner wall of the devices. This is a simple and reproducible method that allows the generation of blood vessel models in vitro.
功能性血管的发展如今是评估新疗法和诊断试剂的一个基本支柱。本文描述了一种圆形截面微流控装置的制造及其通过细胞培养进行的后续功能化。其目的是模拟血管以测试肺动脉高压的新疗法。制造过程采用了一种方法，即使用圆形截面的导线来确定通道尺寸。为了制造血管，在旋转细胞培养条件下接种细胞，以便在装置内壁获得均匀的细胞接种。这是一种简单且可重复的方法，能够在体外生成血管模型。
