Laboratoire de Biologie Moléculaire Et de Génétique, Université Joseph KI-ZERBO, P.O. Box 7021, Ouagadougou 03, Burkina Faso.
Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso.
Mol Biol Rep. 2023 Jun;50(6):5039-5047. doi: 10.1007/s11033-023-08353-0. Epub 2023 Apr 26.
Hepatitis B Virus (HBV) infection affect all social strata of humanity and in the absence of any management, this infection has a different outcome from one infected person to another. This suggests that there are specific individual factors that influence the outcome of the pathology. Sex, immunogenetics and age of contraction of the virus have been cited as factors that influence the evolution of the pathology. In this study, we looked at two alleles of the Human Leucocyte Antigen (HLA) system to measure their possible involvement in the evolution of HBV infection.
We conducted a cohort study involving 144 individuals spread over 04 distinct stages of infection and then compared allelic frequencies in these populations. A multiplex PCR was conducted and the data obtained was analyzed using R and SPSS software. Our study revealed a predominance of HLA-DRB112 in our study population without, however, showing a significant difference between HLA-DRB111 and HLA-DRB112. The HLA-DRB112 proportion was significantly higher in chronic hepatitis B (CHB) and resolved hepatitis B (RHB) compared to cirrhosis and hepatocellular carcinoma (HCC) (p-value = 0,002). Carrying HLA-DRB112 has been associated with a low risk of complication of infection (CHB → cirrhosis; OR 0,33 p-value 0,017; RHB → HCC OR 0,13; p-value = 0,00,045) whereas the presence of HLA-DRB111 in the absence of HLA-DRB1*12 increased the risk of developing severe liver disease. However, a strong interaction of these alleles with the environment could modulate the infection.
Our study shown that HLA-DRB1*12 is the most frequent and it's carriage may be protective in the development of infection.
乙型肝炎病毒（HBV）感染影响人类的所有社会阶层，在没有任何管理的情况下，这种感染在一个感染者和另一个感染者之间有不同的结果。这表明存在影响病理学结果的特定个体因素。性别、免疫遗传学和病毒感染的年龄已被认为是影响病理学演变的因素。在这项研究中，我们研究了人类白细胞抗原（HLA）系统的两个等位基因，以衡量它们在 HBV 感染演变中的可能作用。
我们进行了一项队列研究，涉及 144 名分布在感染的 04 个不同阶段的个体，然后比较了这些人群中的等位基因频率。进行了多重 PCR，并用 R 和 SPSS 软件分析了获得的数据。我们的研究表明，在我们的研究人群中 HLA-DRB112 占主导地位，但 HLA-DRB111 和 HLA-DRB112 之间没有显著差异。与肝硬化和肝细胞癌（HCC）相比，慢性乙型肝炎（CHB）和已解决的乙型肝炎（RHB）中 HLA-DRB112 的比例显著更高（p 值=0.002）。携带 HLA-DRB112 与感染并发症的风险降低相关（CHB→肝硬化；OR 0.33，p 值 0.017；RHB→HCC，OR 0.13，p 值=0.00045），而在没有 HLA-DRB112 的情况下存在 HLA-DRB1*11 则增加了发生严重肝病的风险。然而，这些等位基因与环境的强烈相互作用可能会调节感染。
我们的研究表明，HLA-DRB1*12 是最常见的，其携带可能对感染的发展具有保护作用。
