Dash Jayalaxmi, Sethi Manisha, Deb Sushanta, Parida Deepti, Kar Salona, Mahapatra Soumendu, Minz Aliva P, Pradhan Biswaranjan, Prasad Punit, Senapati Shantibhusan
Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
Regional Centre for Biotechnology, Faridabad, Haryana, India.
World J Microbiol Biotechnol. 2023 Apr 27;39(7):171. doi: 10.1007/s11274-023-03626-z.
Characterization of new potential probiotics is desirable in the field of research on probiotics for their extensive use in health and disease. Tribes could be an unusual source of probiotics due to their unique food habits and least dependence on medications and consumption of antibiotics. The aim of the present study is to isolate lactic acid bacteria from tribal fecal samples of Odisha, India, and characterize their genetic and probiotic attributes. In this context one of the catalase-negative and Gram-positive isolates, identified using 16S rRNA sequencing as Ligilactobacillus salivarius, was characterized in vitro for its acid and bile tolerance, cell adhesion and antimicrobial properties. The whole genome sequence was obtained and analyzed for strain level identification, presence of genomic determinants for probiotic-specific features, and safety. Genes responsible for its antimicrobial and immunomodulatory functions were detected. The secreted metabolites were analyzed using high resolution mass spectroscopy; the results indicated that the antimicrobial potential could be due to the presence of pyroglutamic acid, propionic acid, lactic acid, 2-hydroxyisocaproic acid, homoserine, and glutathione, and the immuno-modulating activity, contributed by the presence of short chain fatty acids such as acetate, propionate, and butyrate. So, to conclude we have successfully characterized a Ligilactobacillus salivarius species with potential antimicrobial and immunomodulatory ability. The health-promoting effects of this probiotic strain and/or its derivatives will be investigated in future.
在益生菌研究领域，对新型潜在益生菌进行特性描述很有必要，因为它们在健康与疾病方面有广泛应用。部落人群可能是益生菌的一个特殊来源，这是由于他们独特的饮食习惯以及对药物和抗生素使用的最少依赖。本研究的目的是从印度奥里萨邦部落人群的粪便样本中分离乳酸菌，并对其遗传和益生菌特性进行表征。在此背景下，其中一株过氧化氢酶阴性且革兰氏阳性的分离株，经16S rRNA测序鉴定为唾液利基乳杆菌，对其体外的耐酸和耐胆汁能力、细胞黏附及抗菌特性进行了表征。获得了全基因组序列并进行分析，以进行菌株水平鉴定、确定益生菌特异性特征的基因组决定因素以及安全性。检测到了负责其抗菌和免疫调节功能的基因。使用高分辨率质谱对分泌的代谢产物进行了分析；结果表明，抗菌潜力可能归因于焦谷氨酸、丙酸、乳酸、2-羟基异己酸、高丝氨酸和谷胱甘肽的存在，而免疫调节活性则由乙酸盐、丙酸盐和丁酸盐等短链脂肪酸的存在所贡献。所以，总之，我们成功地表征了一种具有潜在抗菌和免疫调节能力的唾液利基乳杆菌。这种益生菌菌株和/或其衍生物的健康促进作用将在未来进行研究。
