Laurent Agneta J, Bindslev Niels, Vukojević Vladana, Terenius Lars
Department of Clinical Neuroscience, Center for Molecular Medicine, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark.
ACS Bio Med Chem Au. 2021 Sep 7;1(1):11-20. doi: 10.1021/acsbiomedchemau.1c00017. eCollection 2021 Dec 15.
With the increasing popularity of nonalcoholic beer, the association between beer drinking and alcohol intake is lost. In the present study, we show that nonalcoholic beer can stimulate the expansion of neuron-like cell lines and neuroepithelial stem cells in culture, yielding an effect comparable to that of alcoholic beer. One ingredient in beer is hops, which is derived from the flower of hop plants. The female flower contains humulones, which are transformed into iso-α-acids during wort boiling and give beer its bitter taste. In this study, we tested the effects of these iso-α-acids and/or alcohol on the proliferation of neuron-like cells and neuroepithelial stem cells in culture. Iso-α-acids enhanced cell expansion, showing a bimodal dose-response curve with peaks around 2-30 nM and 2-5 μM, of which nanomolar concentrations are relevant in beer drinking. The more lipophilic -iso-α-acids, found to a greater extent in beer foam, are even more potent. Our results indicate that iso-α-acids, acting via peroxisome proliferator-activated receptors could be responsible for the observed effects. Altogether, our results indicate that nonalcoholic beer with ingredients such as iso-α-acids stimulate the proliferation of neuroepithelial stem cells.
随着无酒精啤酒越来越受欢迎，饮用啤酒与酒精摄入之间的关联已不复存在。在本研究中，我们发现无酒精啤酒能够刺激培养中的神经元样细胞系和神经上皮干细胞的扩增，其效果与酒精性啤酒相当。啤酒中的一种成分是啤酒花，它源自啤酒花植物的花朵。雌花含有葎草酮，在麦芽汁煮沸过程中会转化为异α -酸，赋予啤酒苦味。在本研究中，我们测试了这些异α -酸和/或酒精对培养中的神经元样细胞和神经上皮干细胞增殖的影响。异α -酸促进细胞扩增，呈现双峰剂量反应曲线，峰值分别在2 - 30 nM和2 - 5 μM左右，其中纳摩尔浓度与饮用啤酒相关。在啤酒泡沫中含量更高的亲脂性更强的异α -酸，作用甚至更强。我们的结果表明，通过过氧化物酶体增殖物激活受体起作用的异α -酸可能是造成上述效应的原因。总之，我们的结果表明，含有异α -酸等成分的无酒精啤酒会刺激神经上皮干细胞的增殖。
