Rizk Fatma H, El-Saka Mervat H, Ibrahim Rowida Raafat, El-Deeb Omnia Safwat, Ibrahim Hoda A, El Saadany Amira A, Mashal Shaimaa S, Ammar Leila, Abdelsattar Amal M, Barhoma Ramez A
Department of Physiology, Faculty of Medicine, Tanta University, Egypt.
Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Tanta University, Egypt.
Biofactors. 2023 Jul-Aug;49(4):928-939. doi: 10.1002/biof.1955. Epub 2023 Apr 27.
This study evaluated possible mitigating effect of adropin on lung injury in diabetic rats, targeting role of Rho A/Rho-associated kinase pathway. Rats were allocated into four groups: control, adropin, diabetic, and diabetic+adropin groups. At the termination of the experiment, serum fasting glucose, insulin and adropin levels and insulin resistance were calculated. Wet/dry ratio, histopathological, immunohistochemical analyses, and relative real time gene expression of lung tissue was determined. Interleukin-6, tumor necrosis factor alpha, malondialdehyde, 8-Oxo-2'-deoxyguanosine, reduced glutathione, superoxide dismutase, Bcl-2, BAX, myeloperoxidase, intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and transforming growth factor-β were determined in lung tissue. Adropin treatment in diabetic rats notably attenuated hyperglycemia and insulin resistance. Also, it mitigated diabetic lung injury via suppressing effect on Rho A/ROCK pathway, apoptosis, inflammatory reactions, oxidative stress, and fibrosis of lung tissue. Adropin can be considered as a promising therapeutic agent for treating diabetic lung injury.
本研究评估了内脂素对糖尿病大鼠肺损伤的可能缓解作用，针对Rho A/ Rho相关激酶通路的作用。大鼠被分为四组：对照组、内脂素组、糖尿病组和糖尿病+内脂素组。实验结束时，计算血清空腹血糖、胰岛素和内脂素水平以及胰岛素抵抗。测定肺组织的湿/干比、组织病理学、免疫组织化学分析以及相对实时基因表达。测定肺组织中的白细胞介素-6、肿瘤坏死因子α、丙二醛、8-氧代-2'-脱氧鸟苷、还原型谷胱甘肽、超氧化物歧化酶、Bcl-2、BAX、髓过氧化物酶、细胞间黏附分子-1、血管细胞黏附分子-1和转化生长因子-β。糖尿病大鼠接受内脂素治疗可显著减轻高血糖和胰岛素抵抗。此外，它通过对肺组织的Rho A/ROCK通路、细胞凋亡、炎症反应、氧化应激和纤维化的抑制作用减轻糖尿病肺损伤。内脂素可被视为治疗糖尿病肺损伤的一种有前景的治疗药物。
