College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Toxins (Basel). 2023 Apr 17;15(4):292. doi: 10.3390/toxins15040292.
Ochratoxin A (OTA), as a common mycotoxin, has seriously harmful effects on agricultural products, livestock and humans. There are reports on the regulation of SakA in the MAPK pathway, which regulates the production of mycotoxins. However, the role of SakA in the regulation of and OTA production is not clear. In this study, a deletion mutant () was constructed. The effects of different concentrations of D-sorbitol, NaCl, Congo red and HO on the mycelia growth, conidia production and biosynthesis of OTA were investigated in WT and . The results showed that 100 g/L NaCl and 3.6 M D-sorbitol significantly inhibited mycelium growth and that a concentration of 0.1% Congo red was sufficient to inhibit the mycelium growth. A reduction in mycelium development was observed in , especially in high concentrations of osmotic stress. A lack of dramatically reduced OTA production by downregulating the expression of the biosynthetic genes , , and . However, and the transcription factor 1 were slightly upregulated by 80 g/L NaCl and 2.4 M D-sorbitol, whereas they were downregulated by 0.1% Congo red and 2 mM HO. Furthermore, showed degenerative infection ability toward pears and grapes. These results suggest that is involved in the regulation of fungal growth, OTA biosynthesis and the pathogenicity of and could be influenced by specific environmental stresses.
赭曲霉毒素 A（OTA）作为一种常见的真菌毒素，对农产品、畜牧业和人类健康具有严重的危害作用。有报道称 SakA 调节丝裂原活化蛋白激酶（MAPK）通路，从而调节真菌毒素的产生。然而，SakA 在调控 和 OTA 产生中的作用尚不清楚。在本研究中，构建了一个 SakA 缺失突变体（ ）。在 WT 和 中，研究了不同浓度的 D-山梨醇、NaCl、刚果红和 H2O2 对菌丝生长、分生孢子产生和 OTA 生物合成的影响。结果表明，100 g/L NaCl 和 3.6 M D-山梨醇显著抑制菌丝生长，而 0.1%的刚果红足以抑制菌丝生长。 在高渗透压胁迫下，菌丝发育明显减少。缺失 显著降低 OTA 的产生，下调生物合成基因 、 、 和 的表达。然而，80 g/L NaCl 和 2.4 M D-山梨醇略微上调 和转录因子 1 的表达，而 0.1%的刚果红和 2 mM H2O2 则下调其表达。此外， 对梨和葡萄的侵染能力明显减弱。这些结果表明，SakA 参与了真菌生长、OTA 生物合成以及 和 的致病性的调控，并且可能受到特定环境胁迫的影响。
Zotero 插件
