Lehner T, Mehlert A, Caldwell J
Infect Immun. 1986 Jun;52(3):682-7. doi: 10.1128/iai.52.3.682-687.1986.
Local gingival immunization was attempted in an effort to confine the immune response to the oral cavity and bypass the systemic immune response. A low-molecular-weight (3.8K) streptococcal antigen (SA) I/II was applied 10 times over a period of 1 year to the gingival crevices of rhesus monkeys. The antigen was maintained in situ by means of silicone rubber appliances. Serial examinations over a period of 1 year showed that topical gingival immunization with the 3.8K SA results in a significantly lower incidence of dental caries and colonization of Streptococcus mutans compared with that of the sham-immunized controls. This was associated with an increase in gingival crevicular immunoglobulin G and salivary immunoglobulin A anti-SA I/II antibodies, whereas no change occurred in serum antibodies to SA I/II. The immune mechanism which prevents the colonization of S. mutans and the development of caries may involve antibodies that prevent the adherence of S. mutans to the teeth and facilitate phagocytosis and killing by the local neutrophils. This novel route of local immunization is noninvasive, does not cause side effects, and bypasses systemic immunization.
尝试进行局部牙龈免疫，以将免疫反应局限于口腔并绕过全身免疫反应。在1年的时间里，将低分子量（3.8K）的链球菌抗原（SA）I/II应用于恒河猴的牙龈沟10次。通过硅橡胶装置将抗原原位保留。1年期间的系列检查表明，与假免疫对照组相比，用3.8K SA进行局部牙龈免疫可使龋齿发病率和变形链球菌定植率显著降低。这与龈沟液免疫球蛋白G和唾液免疫球蛋白A抗SA I/II抗体增加有关，而血清抗SA I/II抗体无变化。防止变形链球菌定植和龋齿发生的免疫机制可能涉及阻止变形链球菌黏附于牙齿并促进局部中性粒细胞吞噬和杀伤的抗体。这种新型局部免疫途径是非侵入性的，不会引起副作用，并且绕过全身免疫。
