Tang Yingping, Yu Pan, Chen Lanming
Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Foods. 2023 Apr 13;12(8):1640. doi: 10.3390/foods12081640.
The increase in bacterial resistance and the decline in the effectiveness of antimicrobial agents are challenging issues for the control of infectious diseases. Traditional Chinese herbal plants are potential sources of new or alternative medicine. Here, we identified antimicrobial components and action modes of the methanol-phase extract from an edible herb Wight et Arn, which had a 68.18% inhibition rate against 22 species of common pathogenic bacteria. The extract was purified using preparative high-performance liquid chromatography (Prep-HPLC), and three separated fragments (Fragments 1-3) were obtained. Fragment 1 significantly elevated cell surface hydrophobicity and membrane permeability but reduced membrane fluidity, disrupting the cell integrity of the Gram-negative and Gram-positive pathogens tested ( < 0.05). Sixty-six compounds in Fragment 1 were identified using Ultra-HPLC and mass spectrometry (UHPLC-MS). The identified oxymorphone (6.29%) and rutin (6.29%) were predominant in Fragment 1. Multiple cellular metabolic pathways were altered by Fragment 1, such as the repressed ABC transporters, protein translation, and energy supply in two representative Gram-negative and Gram-positive strains ( < 0.05). Overall, this study demonstrates that Fragment 1 from Wight et Arn is a promising candidate for antibacterial medicine and food preservatives.
细菌耐药性的增加以及抗菌药物有效性的下降是控制传染病面临的挑战性问题。传统中草药是新的或替代药物的潜在来源。在此，我们鉴定了一种可食用草本植物白花败酱甲醇相提取物的抗菌成分和作用模式，该提取物对22种常见病原菌的抑制率为68.18%。通过制备型高效液相色谱（Prep-HPLC）对提取物进行纯化，得到了三个分离片段（片段1-3）。片段1显著提高了细胞表面疏水性和膜通透性，但降低了膜流动性，破坏了所测试的革兰氏阴性和革兰氏阳性病原菌的细胞完整性（P<0.05）。使用超高效液相色谱和质谱（UHPLC-MS）鉴定了片段1中的66种化合物。鉴定出的羟吗啡酮（6.29%）和芦丁（6.29%）在片段1中占主导地位。片段1改变了多种细胞代谢途径，如在两种代表性的革兰氏阴性和革兰氏阳性菌株中抑制ABC转运蛋白、蛋白质翻译和能量供应（P<0.05）。总体而言，本研究表明白花败酱的片段1是抗菌药物和食品防腐剂的有前途的候选物。
