Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530004, China.
Genes (Basel). 2023 Apr 19;14(4):943. doi: 10.3390/genes14040943.
Research on the chloroplast genome of parasitic plants is limited. In particular, the homology between the chloroplast genomes of parasitic and hyperparasitic plants has not been reported yet. In this study, three chloroplast genomes of (, , and ) and one chloroplast genome of () were sequenced and analyzed, among which is the host of . The chloroplast genomes of the four species were 119,941-138,492 bp in length. Compared with the chloroplast genome of the autotrophic plant , all of the genes, three ribosomal protein genes, three tRNA genes and the gene were lost in the three species. Meanwhile, in , the gene and the gene were lost, and only one gene () existed. The results of homology analysis showed that the homology between and its host was low, indicating that grows on its host but they do not share the chloroplast genome. In addition, horizontal gene transfer was not found between and its host . Several candidate highly variable regions in the chloroplast genomes of and species were selected for species identification study. Phylogenetic analysis revealed that the species of and were closely related and supported that and should be treated as congeneric, while species in had a close relationship with that in .
寄生植物的叶绿体基因组研究较少。特别是，寄生和超寄生植物的叶绿体基因组之间的同源性尚未报道。本研究对 3 种寄生植物（ 、 、 ）和 1 种超寄生植物（ ）的叶绿体基因组进行了测序和分析，其中 是 的宿主。这 4 个物种的叶绿体基因组长度分别为 119941-138492bp。与自养植物 相比，3 种 物种的叶绿体基因组均丢失了 基因、3 个核糖体蛋白基因、3 个 tRNA 基因和 基因。同时， 在 基因和 基因丢失，仅存在一个 基因（ ）。同源性分析结果表明， 与其宿主 的同源性较低，表明 生长在其宿主 上，但它们不共享叶绿体基因组。此外， 在与其宿主 之间未发现水平基因转移。选择 和 物种的叶绿体基因组中的几个候选高度可变区进行物种鉴定研究。系统发育分析表明， 和 的物种密切相关，支持 和 应被视为同属，而 中的物种与 中的物种关系密切。
