使用脲柱前衍生化的 LC-MS 进行氨基酸分析的实用方法。
A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
机构信息
Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China.
Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo 315211, China.
出版信息
Int J Mol Sci. 2023 Apr 15;24(8):7332. doi: 10.3390/ijms24087332.
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.
氨基酸（AA）分析在生物化学、食品科学和临床医学中都很重要。然而，由于其内在的局限性，AA 通常需要衍生化以改善其分离和测定。在这里，我们提出了一种使用简单试剂尿素对 AA 进行衍生化的液相色谱-质谱（LC-MS）方法。在没有任何预处理步骤的情况下，这些反应在很宽的条件范围内都能定量进行。与未衍生化的产物相比，20 种 AA 的尿素衍生化产物（氨甲酰氨基酸）在反相柱上的分离效果更好，在紫外检测器中的响应也增强了。我们将这种方法应用于以细胞培养介质为模型的复杂样品中的 AA 分析，显示出了对寡肽测定的潜力。这种快速、简单和廉价的方法应该对复杂样品中的 AA 分析有用。
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