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G 蛋白 β 亚基基因增强小麦的抗旱性和耐盐性。

G-Protein β-Subunit Gene Enhances Drought and Salt Resistance in Wheat.

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China.

出版信息

Int J Mol Sci. 2023 Apr 15;24(8):7337. doi: 10.3390/ijms24087337.

Abstract

In the hexaploid wheat genome, there are three Gα genes, three Gβ and twelve Gγ genes, but the function of Gβ in wheat has not been explored. In this study, we obtained the overexpression of Arabidopsis plants through inflorescence infection, and the overexpression of wheat lines was obtained by gene bombardment. The results showed that under drought and NaCl treatment, the survival rate of Arabidopsis seedlings' overexpression of was higher than that of the wild type, while the survival rate of the related mutant was lower than that of the wild type. The survival rate of wheat seedlings with overexpression was higher than that of the control. In addition, under drought and salt stress, the levels of superoxide dismutase (SOD) and proline (Pro) in the wheat overexpression of were higher than that of the control, and the concentration of malondialdehyde (MDA) was lower than that of the control. This indicates that could improve the drought resistance and salt tolerance of Arabidopsis and wheat by scavenging active oxygen. Overall, this work provides a theoretical basis for wheat G-protein β-subunits in a further study, and new genetic resources for the cultivation of drought-tolerant and salt-tolerant wheat varieties.

摘要

在六倍体小麦基因组中,存在三个 Gα 基因、三个 Gβ 和十二个 Gγ 基因,但 Gβ 在小麦中的功能尚未得到探索。本研究通过花序浸染获得拟南芥植株的过表达,通过基因枪轰击获得小麦品系的过表达。结果表明,在干旱和 NaCl 处理下,拟南芥幼苗过表达 的存活率高于野生型,而相关突变体 的存活率低于野生型。过表达 的小麦幼苗的存活率高于对照。此外,在干旱和盐胁迫下,过表达 的小麦中超氧化物歧化酶(SOD)和脯氨酸(Pro)的水平高于对照,丙二醛(MDA)的浓度低于对照。这表明 通过清除活性氧可以提高拟南芥和小麦的抗旱性和耐盐性。总的来说,这项工作为小麦 G 蛋白 β 亚基的进一步研究提供了理论依据,为培育抗旱耐盐小麦品种提供了新的遗传资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e542/10138664/17c97b1c3e4c/ijms-24-07337-g001.jpg

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