The Prevalence of Genital Mycoplasmas and Coinfection with in Female Patients in Vienna, Austria.

causes trichomoniasis, the most recurrent sexually transmitted infection (STI) worldwide. Genital mycoplasmas, not considered STI agents, are frequently isolated from the female genital tract. A symbiosis between species and has been described. The aim of this study was to conduct molecular-based analyses of vaginal specimens, thus assessing the prevalence of non-STI infections. In total, 582 samples from female patients and an additional 20 isolates were analyzed by PCR using specific 16S rRNA primers, and the obtained PCR products were sequenced. species were detected in 28.2% of the collected vaginal samples. was found in 21.5% of the specimens, species were found in 7.5% of the samples. The molecular data of the newly described species, , were obtained for the first time in Austria, in a sample also positive for . Analyses of the cultivated strains confirmed the presence of in two out of 20 samples. A comparably high prevalence of genital mycoplasmas was revealed through advanced diagnostic assays, with and being the most prevalent species. The previously described symbiotic relationship between and was confirmed.