Intitute for Specific Prophylaxis and Tropical Medicine (ISPTM), Centre for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
Pilzambulatorium Schloesselgasse, Oupatients Centre for Diagnosis of Venero-Dermatological Diseases, 1080 Vienna, Austria.
Int J Mol Sci. 2022 Oct 17;23(20):12422. doi: 10.3390/ijms232012422.
(TV) is the causative agent of trichomoniasis, the most common nonviral sexually transmitted disease. TV can carry symbionts such as virus (TVV) or . Four distinct strains of TV are known: TVV1, TVV2, TVV3, and TVV4. The aim of the current study was to characterise TV isolates from Austrian patients for the presence of symbionts, and to determine their effect on metronidazole susceptibility and cytotoxicity against HeLa cells. We collected 82 TV isolates and detected presence of TVV (TVV1, TVV2, or TVV3) in 29 of them (35%); no TVV4 was detected. was detected in vaginal/urethral swabs by culture in 37% of the TV-positive patients; DNA was found in 28% of the TV isolates by PCR. In 15% of the patients, was detected in the clinical samples as well as within the respective TV isolates. In 22% of the patients, was detected by culture only. In 11 patients, was detected only within the respective cultured TV isolates (13%), while the swab samples were negative for . Our results provide a first insight into the distribution of symbionts in TV isolates from Austrian patients. We did not observe significant effects of the symbionts on metronidazole susceptibility, cytotoxicity, or severity of symptoms.
（TV）是滴虫病的病原体，这是最常见的非病毒性性传播疾病。TV 可以携带共生体，如病毒（TVV）或。已知有四种不同的 TV 株：TVV1、TVV2、TVV3 和 TVV4。本研究的目的是对来自奥地利患者的 TV 分离株进行共生体的特征描述，并确定它们对甲硝唑敏感性和对 HeLa 细胞的细胞毒性的影响。我们收集了 82 个 TV 分离株，并在其中 29 个（35%）中检测到 TVV（TVV1、TVV2 或 TVV3）的存在；未检测到 TVV4。通过培养在 37%的 TV 阳性患者的阴道/尿道拭子中检测到；通过 PCR 在 28%的 TV 分离株中发现了 DNA。在 15%的患者中，在临床样本以及各自的 TV 分离株中均检测到。在 22%的患者中，仅通过培养检测到。在 11 名患者中，仅在各自培养的 TV 分离株中（13%）检测到，而拭子样本对呈阴性。我们的结果首次提供了奥地利患者的 TV 分离株中共生体分布的见解。我们没有观察到共生体对甲硝唑敏感性、细胞毒性或症状严重程度有显著影响。
