Farlow Anthony J, Rupasinghe Darshani B, Naji Khalid M, Capon Robert J, Spiteller Dieter
Chemical Ecology/Biological Chemistry, Department of Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Centre for Drug Discovery, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, Brisbane, QLD 4072, Australia.
Microorganisms. 2023 Apr 12;11(4):1005. doi: 10.3390/microorganisms11041005.
bacteria have been previously isolated predominantly from floral nectar and identified in metagenomic screenings as associated with bees. Here, we isolated three strains from the robust Australian stingless bee sharing over 99.4% sequence similarity with strains isolated from floral nectar. The three strains (D21B, D08K, D15G) from exhibited near-identical 16 rDNA. The genome of strain D21B was sequenced; its draft genome contains 3,294,717 bp, with a GC content of 47.38%. Genome annotation revealed 3236 protein-coding genes. The genome of D21B differs sufficiently from the closest related strain, 2.1A, to constitute a new species. In contrast to 2.1A, strain D21B produces the volatile 2-phenylethanol. The D21B genome contains a polyketide/non-ribosomal peptide gene cluster not present in any other draft genomes. Moreover, the strains isolated from grew in a minimal medium without thiamine, but 2.1A was thiamine-dependent. Strain D21B was named D21B, reflecting its origin from stingless bees. strains may contribute to the fitness of
此前，细菌主要是从花蜜中分离出来的，并在宏基因组筛选中被鉴定为与蜜蜂有关。在这里，我们从强壮的澳大利亚无刺蜂中分离出三株菌株，它们与从花蜜中分离出的菌株序列相似性超过99.4%。这三株菌株（D21B、D08K、D15G）的16 rDNA近乎相同。对菌株D21B的基因组进行了测序；其草图基因组包含3,294,717 bp，GC含量为47.38%。基因组注释显示有3236个蛋白质编码基因。D21B的基因组与最接近的相关菌株2.1A有足够差异，可以构成一个新物种。与2.1A不同，菌株D21B产生挥发性的2-苯乙醇。D21B基因组包含一个聚酮化合物/非核糖体肽基因簇，在任何其他草图基因组中都不存在。此外，从[此处原文缺失相关内容]分离出的菌株能在不含硫胺素的基本培养基中生长，但2.1A依赖硫胺素。菌株D21B被命名为[此处原文缺失相关内容]D21B，表示其源自无刺蜂。[此处原文缺失相关内容]菌株可能有助于[此处原文缺失相关内容]的适应性
