Laviad-Shitrit Sivan, Izhaki Ido, Whitman William B, Shapiro Nicole, Woyke Tanja, Kyrpides Nikos C, Halpern Malka
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel.
Department of Microbiology, University of Georgia, Athens, GA, USA.
PeerJ. 2020 Apr 6;8:e8822. doi: 10.7717/peerj.8822. eCollection 2020.
strain 8N4, the type species of the genus , was isolated from (almond) floral nectar. Other strains of this species were isolated from the floral nectar of (grapefruit), (tobacco tree) and from strain 8N4 is a Gram-negative, oxidase-negative, facultatively anaerobic bacterium in the family .
Here we describe features of this organism, together with its genome sequence and annotation. The DNA GC content is 47.38%, the assembly size is 3,294,717 bp, and the total number of genes are 3,346. The genome discloses the possible role that this species may play in the plant. The genome contains both virulence genes, like pectin lyase and hemolysin, that may harm plant cells and genes that are predicted to produce volatile compounds that may impact the visitation rates by nectar consumers, such as pollinators and nectar thieves.
The genome of strain 8N4 reveals a mutualistic interaction with the plant host and a possible effect on plant pollination and fitness.
8N4菌株是该属的模式种，从（杏仁）花蜜中分离得到。该物种的其他菌株从（葡萄柚）、（烟草树）的花蜜中以及从8N4菌株中分离得到，8N4菌株是肠杆菌科的革兰氏阴性、氧化酶阴性、兼性厌氧菌。
在此我们描述了该生物体的特征，以及其基因组序列和注释。DNA的GC含量为47.38%，组装大小为3,294,717 bp，基因总数为3,346个。该基因组揭示了该物种在植物中可能发挥的作用。基因组中既包含可能损害植物细胞的毒力基因，如果胶裂解酶和溶血素，也包含预计会产生挥发性化合物的基因，这些化合物可能会影响花蜜消费者（如传粉者和花蜜窃贼）的访问率。
8N4菌株的基因组揭示了与植物宿主的互利共生相互作用以及对植物授粉和适应性的可能影响。
