Ghorbani Tajani Anahita, Bisha Bledar
Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA.
Microorganisms. 2023 Apr 14;11(4):1029. doi: 10.3390/microorganisms11041029.
Listeriosis outbreaks were associated with contaminated fruits and vegetables, including cantaloupe, apples, and celery. Grape seed extract (GSE) is a natural antimicrobial with potential for reducing contamination in food. This study assessed the effectiveness of GSE to reduce on fresh produce and the impact of food matrices on its antilisterial activity. GSE showed MIC values of 30-35 μg/mL against four strains used in this study. A total of 100 g portions of cantaloupe, apples, and celery were inoculated with and treated with 100-1000 μg/mL of GSE for 5 or 15 min. Results were analyzed using Rstudio and a Tukey's test. Treated produce had significantly lower counts than the control samples (-value < 0.05). The inhibition was significantly higher on apples and lowest on cantaloupe. Moreover, a 15 min treatment was found to be more effective than a 5 min treatment in reducing on all produce types. The reduction in levels varied between 0.61 and 2.5 log CFU reduction, depending on the treatment concentration, duration, and produce matrix. These findings suggest that GSE is an effective antilisterial treatment for fresh produce, with varying levels of effectiveness depending on the food matrix and treatment time.
李斯特菌病疫情与受污染的水果和蔬菜有关，包括哈密瓜、苹果和芹菜。葡萄籽提取物（GSE）是一种天然抗菌剂，具有降低食品污染的潜力。本研究评估了GSE对新鲜农产品上李斯特菌的减少效果以及食品基质对其抗李斯特菌活性的影响。GSE对本研究中使用的四种李斯特菌菌株的最低抑菌浓度（MIC）值为30 - 35μg/mL。将100克的哈密瓜、苹果和芹菜分别接种李斯特菌，并用100 - 1000μg/mL的GSE处理5或15分钟。使用Rstudio和Tukey检验对结果进行分析。处理后的农产品李斯特菌计数显著低于对照样品（P值<0.05）。对苹果的抑制作用显著高于哈密瓜，对哈密瓜的抑制作用最低。此外，发现在所有农产品类型上，15分钟的处理在减少李斯特菌方面比5分钟的处理更有效。根据处理浓度、持续时间和农产品基质的不同，李斯特菌水平的降低幅度在0.61至2.5个对数CFU减少之间。这些发现表明，GSE是一种有效的新鲜农产品抗李斯特菌处理方法，其有效性水平因食品基质和处理时间而异。
