Leis Eric M, Dziki Sara, Standish Isaac, Waller Diane, Richard Jordan, Weinzinger Jesse, Harris Cleyo, Knowles Susan, Goldberg Tony
La Crosse Fish Health Center-Midwest Fisheries Center, U.S. Fish and Wildlife Service, Onalaska, WI 54650, USA.
U.S. Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, WI 54603, USA.
Microorganisms. 2023 Apr 19;11(4):1068. doi: 10.3390/microorganisms11041068.
Recent bacteriological investigations of freshwater mussel mortality events in the southeastern United States have identified a variety of bacteria and differences in bacterial communities between sick and healthy mussels. In particular, and spp. have been shown to be associated with moribund mussels, although it remains unclear whether these bacteria are causes or consequences of disease. To further understand the role of bacteria in mussel epizootics, we investigated mortality events that occurred in the upper Midwest in the Embarrass River (Wisconsin) and the Huron River (Michigan). For comparison, we also studied mussels from an unaffected population in the St. Croix River (Wisconsin). Diverse bacterial genera were identified from these sites, including from moribund mussels in the Embarrass River (Wisconsin). This bacterium has also been consistently isolated during ongoing mortality events in the Clinch River (Virginia). Subsequently, we developed and validated molecular assays for the detection of to use in future investigations of mussel mortality events and to identify environmental reservoirs of this bacterium.
近期对美国东南部淡水贻贝死亡事件的细菌学调查，已识别出多种细菌以及患病和健康贻贝之间细菌群落的差异。特别是，[细菌名称1]和[细菌名称2]已被证明与濒死贻贝有关，尽管尚不清楚这些细菌是疾病的病因还是结果。为了进一步了解细菌在贻贝流行病中的作用，我们调查了发生在中西部上游的埃姆巴拉斯河（威斯康星州）和休伦河（密歇根州）的死亡事件。作为比较，我们还研究了来自圣克罗伊河（威斯康星州）未受影响种群的贻贝。从这些地点识别出了多种细菌属，包括来自埃姆巴拉斯河（威斯康星州）濒死贻贝的[细菌名称3]。在克林奇河（弗吉尼亚州）持续的死亡事件中也一直分离出这种细菌。随后，我们开发并验证了用于检测[细菌名称3]的分子检测方法，以便在未来对贻贝死亡事件的调查中使用，并识别这种细菌的环境储存库。
