通过将高活性含钴多钨酸盐在聚合离子液体上固化制备基于多金属氧酸盐的复合材料，用于蛋白酶 K 的有效分离。
Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K.
机构信息
Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
Bayuquan Customs District of the People's Republic of China, Yingkou 115007, China.
出版信息
Molecules. 2023 Apr 7;28(8):3307. doi: 10.3390/molecules28083307.
A novel porous polyoxometalate (POM)-based composite (CoPW-PDDVAC) was prepared via the solidification of water-soluble polytungstate (CoPW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co of the CoPW and the aspartic acid residues of proteinase K endowed the obtained CoPW-PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g. The CoPW-PDDVAC composite was applied in the selective isolation of highly active proteinase K from Limber crude enzyme fluid.
一种新型的多金属氧酸盐（POM）基复合材料（CoPW-PDDVAC）通过水溶性多钨酸盐（CoPW）在聚合离子液体二甲基十二烷基-4-聚乙烯苄基氯化铵（PDDVAC）上的固相结合，通过阳离子交换反应。固化通过 EDS、SEM、FT-IR、TGA 等进行确认。高活性 CoPW 中的 Co 与蛋白酶 K 中的天门冬氨酸残基之间的强共价配位和氢键相互作用赋予所得 CoPW-PDDVAC 复合材料优异的蛋白酶 K 吸附性能。热力学研究表明，蛋白酶 K 的吸附行为符合线性 Langmuir 等温模型，吸附容量高达 1428mg/g。CoPW-PDDVAC 复合材料用于从 Limber 粗酶液中选择性分离高活性的蛋白酶 K。
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