Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
Bayuquan Customs District of the People's Republic of China, Yingkou 115007, China.
Molecules. 2023 Apr 7;28(8):3307. doi: 10.3390/molecules28083307.
A novel porous polyoxometalate (POM)-based composite (CoPW-PDDVAC) was prepared via the solidification of water-soluble polytungstate (CoPW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co of the CoPW and the aspartic acid residues of proteinase K endowed the obtained CoPW-PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g. The CoPW-PDDVAC composite was applied in the selective isolation of highly active proteinase K from Limber crude enzyme fluid.
一种新型的多金属氧酸盐（POM）基复合材料（CoPW-PDDVAC）通过水溶性多钨酸盐（CoPW）在聚合离子液体二甲基十二烷基-4-聚乙烯苄基氯化铵（PDDVAC）上的固相结合，通过阳离子交换反应。固化通过 EDS、SEM、FT-IR、TGA 等进行确认。高活性 CoPW 中的 Co 与蛋白酶 K 中的天门冬氨酸残基之间的强共价配位和氢键相互作用赋予所得 CoPW-PDDVAC 复合材料优异的蛋白酶 K 吸附性能。热力学研究表明，蛋白酶 K 的吸附行为符合线性 Langmuir 等温模型，吸附容量高达 1428mg/g。CoPW-PDDVAC 复合材料用于从 Limber 粗酶液中选择性分离高活性的蛋白酶 K。
