SEB1741 适体是一种有效的工具，可阻断葡萄球菌肠毒素 B 诱导的 CD4+T 细胞活化。

The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B.

机构信息

Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico.

Laboratory of Medical Bacteriology, Department of Microbiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, Mexico.

出版信息

Molecules. 2023 Apr 14;28(8):3480. doi: 10.3390/molecules28083480.

DOI:10.3390/molecules28083480

PMID:37110712

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10142257/

Abstract

UNLABELLED

Staphylococcal enterotoxin B (SEB) is a protein produced by , which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of action and its potential use as an immune therapy. However, the efficiency of the SEB1741 aptamer in blocking SEB has not been experimentally demonstrated.

METHODS

Enrichment CD4+ T cells were stimulated with SEB, and as a blocker, we used the SEB1741 aptamer, which was previously synthesised by an "in silico" analysis, showing high affinity and specificity to SEB. The efficiency of the SEB1741 aptamer in blocking CD4+ T cell activation was compared with that of an anti-SEB monoclonal antibody. Flow cytometry and Bio-Plex were used to evaluate the T-cell function.

RESULTS

In vitro, SEB induced the activation of CD4+ T cells and favoured a Th1 profile; however, the SEB1741 aptamer was highly efficient in decreasing the frequency of CD4+ T cells positive to ki-67 and CD69 cells, this means that proliferation and activation of CD4+ T cells was decreased. Moreover, the production of interleukin 2 (IL-2) and interferon-gamma (IFN-γ) was affected, suggesting that the Th1 profile is not present when the SEB1441 aptamer is used. Thus, the SEB1741 function was similar to that of anti-SEB.

CONCLUSIONS

The SEB1741 aptamer is a valuable tool for blocking CD4+ T cell activation and the subsequent release of proinflammatory cytokines by SEB stimulation.

摘要

未加标签

葡萄球菌肠毒素 B（SEB）是一种由金黄色葡萄球菌产生的蛋白质，对人类有毒。它以刺激促炎 CD4+T 细胞（Th1 表型）的过度激活而闻名，并且已经进行了体外研究来了解其作用机制及其作为免疫疗法的潜在用途。然而，SEB1741 适体阻断 SEB 的效率尚未通过实验证明。

方法

用 SEB 刺激富集的 CD4+T 细胞，作为阻断剂，我们使用了 SEB1741 适体，该适体是通过“计算机分析”预先合成的，对 SEB 具有高亲和力和特异性。SEB1741 适体阻断 CD4+T 细胞活化的效率与抗 SEB 单克隆抗体进行了比较。流式细胞术和 Bio-Plex 用于评估 T 细胞功能。

结果

在体外，SEB 诱导 CD4+T 细胞的激活并有利于 Th1 表型；然而，SEB1741 适体非常有效地降低了 CD4+T 细胞中 ki-67 和 CD69 阳性细胞的频率，这意味着 CD4+T 细胞的增殖和激活减少了。此外，白细胞介素 2（IL-2）和干扰素-γ（IFN-γ）的产生受到影响，表明当使用 SEB1441 适体时不存在 Th1 表型。因此，SEB1741 的功能类似于抗 SEB。