State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
China Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China.
Toxins (Basel). 2023 May 28;15(6):363. doi: 10.3390/toxins15060363.
Exposure to enterotoxin B (SEB) is one of the causes of food poisoning and is associated with several immune diseases due to its superantigen capability. This study aimed to characterize the differentiations of naïve Th cells stimulated with different doses of SEB. The expression of T-bet, GATA-3, and Foxp3 or secretion of IFN-γ, IL-4, IL-5, IL-13, and IL-10 were evaluated in wild-type (WT) or DO11.10 CD4 T cells co-cultured with bone marrow dendritic cells (BMDCs). We found that the balance of Th1/Th2 could be dominated by the doses of SEB stimulation. A higher SEB dose could induce more Th1 and a lower Th2/Th1 ratio in Th cells co-cultured with BMDCs. This different tendency of Th cell differentiation induced by the SEB complements the existing knowledge about SEB acting as a superantigen to activate Th cells. Additionally, it is also helpful in managing the colonization of and food contamination of SEB.
肠毒素 B（SEB）暴露是食物中毒的原因之一，由于其超抗原能力，与几种免疫疾病有关。本研究旨在表征用不同剂量 SEB 刺激的幼稚 Th 细胞的分化。在与骨髓树突状细胞（BMDC）共培养的野生型（WT）或 DO11.10 CD4 T 细胞中评估 T-bet、GATA-3 和 Foxp3 的表达或 IFN-γ、IL-4、IL-5、IL-13 和 IL-10 的分泌。我们发现，Th1/Th2 的平衡可以由 SEB 刺激的剂量来主导。与 BMDC 共培养的 Th 细胞中，较高的 SEB 剂量可诱导更多的 Th1 和较低的 Th2/Th1 比值。SEB 诱导 Th 细胞分化的这种不同趋势补充了 SEB 作为超抗原激活 Th 细胞的现有知识。此外，它还有助于管理 SEB 的定植和食物污染。
