IDiBE, Institute for R&D in Health Biotechnology of Elche, University Miguel Hernández of Elche, Avda. de la Universidad, 03202 Elche, Spain.
Mitra Sol Technologies S.L. Parque Científico y Empresarial UMH, Edificio Quorum III, Avda. de la Universidad, 03202 Elche, Spain.
Molecules. 2023 Apr 18;28(8):3552. doi: 10.3390/molecules28083552.
Gas chromatography (GC) techniques for analyzing and determining the cannabinoid profile in cannabis ( L.) are widely used in standard laboratories; however, these methods may mislabel the profile when used under rapid conditions. Our study aimed to highlight this problem and optimize GC column conditions and mass spectrometry (MS) parameters to accurately identify cannabinoids in both standards and forensic samples. The method was validated for linearity, selectivity, and precision. It was observed that when tetrahydrocannabinol (Δ9-THC) and cannabidiolic acid (CBD-A) were examined using rapid GC conditions, the resulting derivatives generated identical retention times. Wider chromatographic conditions were applied. The linear range for each compound ranged from 0.02 μg/mL to 37.50 μg/mL. The R values ranged from 0.996 to 0.999. The LOQ values ranged from 0.33 μg/mL to 5.83 μg/mL, and the LOD values ranged from 0.11 μg/mL to 1.92 μg/mL. The precision values ranged from 0.20% to 8.10% RSD. In addition, forensic samples were analyzed using liquid chromatography (HPLC-DAD) in an interlaboratory comparison test, with higher CBD and THC content than GC-MS determination ( < 0.05) in samples. Overall, this study highlights the importance of optimizing GC techniques to avoid mislabeling cannabinoids in cannabis samples.
气相色谱（GC）技术广泛应用于标准实验室，用于分析和测定大麻（L.）中的大麻素谱；然而，在快速条件下使用这些方法可能会导致谱图标记错误。我们的研究旨在强调这一问题，并优化 GC 柱条件和质谱（MS）参数，以准确识别标准品和法医样本中的大麻素。该方法经过线性、选择性和精密度验证。结果表明，当使用快速 GC 条件检测四氢大麻酚（Δ9-THC）和大麻二酚酸（CBD-A）时，生成的衍生物具有相同的保留时间。应用更宽的色谱条件。每个化合物的线性范围为 0.02μg/mL 至 37.50μg/mL。R 值范围为 0.996 至 0.999。LOQ 值范围为 0.33μg/mL 至 5.83μg/mL，LOD 值范围为 0.11μg/mL 至 1.92μg/mL。精密度值范围为 0.20%至 8.10%RSD。此外，在实验室间比较测试中，使用高效液相色谱（HPLC-DAD）对法医样本进行分析，结果显示 CBD 和 THC 含量高于 GC-MS 测定（<0.05）。总的来说，这项研究强调了优化 GC 技术以避免大麻样品中大麻素标记错误的重要性。
