从 sp. CB09030 中分离并阐明螺环四烯酮 Lobophorins A、B 和 H8 的结构及其生物合成基因簇。
The Isolation and Structure Elucidation of Spirotetronate Lobophorins A, B, and H8 from sp. CB09030 and Their Biosynthetic Gene Cluster.
机构信息
Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China.
School of Pharmaceutical Sciences, Changsha Health Vocational College, Changsha 410100, China.
出版信息
Molecules. 2023 Apr 20;28(8):3597. doi: 10.3390/molecules28083597.
Lobophorins (LOBs) are a growing family of spirotetronate natural products with significant cytotoxicity, anti-inflammatory, and antibacterial activities. Herein, we report the transwell-based discovery of sp. CB09030 from a panel of 16 in-house strains, which has significant anti-mycobacterial activity and produces LOB A (), LOB B (), and LOB H8 (). Genome sequencing and bioinformatic analyses revealed the potential biosynthetic gene cluster (BGC) for -, which is highly homologous with the reported BGCs for LOBs. However, the glycosyltransferase LobG1 in . sp. CB09030 has certain point mutations compared to the reported LobG1. Finally, LOB analogue (O-β-D-kijanosyl-(1→17)-kijanolide) was obtained through an acid-catalyzed hydrolysis of . Compounds - showed different antibacterial activities against and , which revealed the varying roles of different sugars in their antibacterial activities.
类脂生物碱（LOBs）是一类具有显著细胞毒性、抗炎和抗菌活性的不断增长的螺环四烯天然产物家族。本文报道了从一组 16 株内部菌株中基于 Transwell 的发现，该菌株具有显著的抗分枝杆菌活性，并产生 LOB A()、LOB B()和 LOB H8()。基因组测序和生物信息学分析揭示了潜在的生物合成基因簇（BGC），与报道的 LOBs BGC 高度同源。然而，与报道的 LobG1 相比，在. sp. CB09030 中的糖基转移酶 LobG1 具有某些点突变。最后，通过. 的酸催化水解得到 LOB 类似物()。化合物 - 对 和 显示出不同的抗菌活性，这表明不同糖在其抗菌活性中发挥不同的作用。
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