Department of Anatomy and Cell Biology, Institute of Cancer Research and Translational Medicine, Medical Research Center, University of Oulu, P.O. Box 5000, 90014 Oulu, Finland.
Department of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of Turku, 20520 Turku, Finland.
Molecules. 2023 Apr 21;28(8):3611. doi: 10.3390/molecules28083611.
Hydroxyapatite (HA; Ca(PO)(OH)) coating of bone implants has many beneficial properties as it improves osseointegration and eventually becomes degraded and replaced with new bone. We prepared HA coating on a titanium substrate with atomic layer deposition (ALD) and compared monocyte differentiation and material resorption between ALD-HA and bone. After stimulation with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappa-B ligand (RANKL), human peripheral blood monocytes differentiated into resorbing osteoclasts on bovine bone, but non-resorbing foreign body cells were observed on ALD-HA. The analysis of the topography of ALD-HA and bone showed no differences in wettability (water contact angle on ALD-HA 86.2° vs. 86.7° on the bone), but the surface roughness of ALD-HA (Ra 0.713 µm) was significantly lower compared to bone (Ra 2.30 µm). The cellular reaction observed on ALD-HA might be a consequence of the topographical properties of the coating. The absence of resorptive osteoclasts on ALD-HA might indicate inhibition of their differentiation or the need to modify the coating to induce osteoclast differentiation.
羟基磷灰石（HA；Ca(PO)(OH)）涂层的骨植入物具有许多有益的特性，因为它可以改善骨整合，最终被降解并被新骨取代。我们使用原子层沉积（ALD）在钛基板上制备 HA 涂层，并比较了 ALD-HA 和骨之间的单核细胞分化和材料吸收。在巨噬细胞集落刺激因子（M-CSF）和核因子 kappa-B 配体受体激活剂（RANKL）刺激后，人外周血单核细胞在牛骨上分化为破骨细胞，但在 ALD-HA 上观察到非吸收性异物细胞。对 ALD-HA 和骨的形貌分析表明，润湿性（ALD-HA 上的水接触角为 86.2°，骨上为 86.7°）没有差异，但 ALD-HA 的表面粗糙度（Ra 0.713 µm）明显低于骨（Ra 2.30 µm）。在 ALD-HA 上观察到的细胞反应可能是涂层形貌特性的结果。在 ALD-HA 上没有吸收性破骨细胞可能表明其分化受到抑制，或者需要修饰涂层以诱导破骨细胞分化。
