Zhou Yuxuan, Yu Shuyang, Su Chenjun, Gao Shengqi, Jiang Guilai, Zhou Zhemin, Li Heng
Pasteurien College, Suzhou Medical College of Soochow University, Suzhou 215123, China.
Pathogens. 2023 Apr 2;12(4):549. doi: 10.3390/pathogens12040549.
is an opportunistic pathogen that causes invasive infections in humans. In recent years, increasing studies have focused on the prevalence of infections in adults; however, the epidemiology and molecular characteristics of from Chinese pediatric patients remain unknown. The present study examined the population structure, antimicrobial resistance, and virulent factors of methicillin-resistant and -susceptible isolated from Chinese pediatric patients from one medical center in eastern China. A total of 81 cases were screened with positive infections among 864 pediatric patients between 2016 and 2022 in eastern China. Molecular analysis showed that ST22 (28.4%) and ST59 (13.6%) were the most typical strains, and associations between different clonal complex (CC) types/serotype types (ST) and the age of pediatric patients were observed in this study. CC398 was the predominant type in neonates under 1 month of age, while CC22 was mainly found in term-infant (under 1 year of age) and toddlers (over 1 year of age). Additionally, 17 isolates were resistant to at least three antimicrobials and majority of them belonged to CC59. The gene was found in 59 isolates and gene was present in 26 strains identified as methicillin-resistant. Numerous virulent factors were detected in isolated from present pediatric patients. Remarkably, F-PV and S-PV were dominantly carried by CC22, -1 genes were detected in CC188, CC7, and CC15, while exfoliative toxin genes were found only in CC121. Only 41.98% of the isolates possessed gene, indicating that the sources of infections in pediatric patients may include both human-to-human transmissions as well as environmental and nosocomial infections. Together, the present study provided a phylogenetic and genotypic comparison of from Chinese pediatric patients in Suzhou city. Our results suggested that the colonization of multi-drug resistant isolates of may raise concern among pediatric patients, at least from the present medical center in eastern China.
是一种可导致人类侵袭性感染的机会致病菌。近年来，越来越多的研究聚焦于成人感染的流行情况；然而，来自中国儿科患者的该菌的流行病学和分子特征仍不清楚。本研究检测了从中国东部一家医疗中心的儿科患者中分离出的耐甲氧西林和对甲氧西林敏感的该菌的种群结构、抗菌药物耐药性及毒力因子。2016年至2022年期间，在中国东部对864名儿科患者进行筛查，共有81例该菌感染呈阳性。分子分析表明，ST22（28.4%）和ST59（13.6%）是最典型的菌株，本研究观察到不同克隆复合体（CC）类型/血清型（ST）与儿科患者年龄之间的关联。CC398是1月龄以下新生儿中的主要类型，而CC22主要见于足月儿（1岁以下）和幼儿（1岁以上）。此外，17株该菌分离株对至少三种抗菌药物耐药，其中大多数属于CC59。在59株分离株中发现了该基因，在26株被鉴定为耐甲氧西林的菌株中存在该基因。从目前儿科患者中分离出的该菌检测到众多毒力因子。值得注意的是，F-PV和S-PV主要由CC22携带，在CC188、CC7和CC15中检测到-1基因，而剥脱毒素基因仅在CC121中发现。仅41.98%的该菌分离株拥有该基因，表明儿科患者感染源可能包括人际传播以及环境和医院感染。总之，本研究提供了苏州市中国儿科患者该菌的系统发育和基因型比较。我们的结果表明，该菌多重耐药分离株的定植可能引起儿科患者的关注，至少在中国东部目前的这家医疗中心是这样。
