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中国黄瓜白粉病病原菌分离株YZU573的全基因组序列

A Complete Genome Sequence of Isolate YZU573, the Causal Agent of Powdery Mildew Isolated from Cucumber in China.

作者信息

Wang Ziyi, Du Yujiao, Li Suhao, Xu Xuewen, Chen Xuehao

机构信息

School of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

出版信息

Pathogens. 2023 Apr 6;12(4):561. doi: 10.3390/pathogens12040561.

Abstract

is a well-known obligate biotrophic pathogen that causes powdery mildew (PM) disease on cucurbitaceous plants and is one of the most important limiting factors for cucumber production worldwide. To better understand the avirulence effector proteins in this species that are known to be involved in host-pathogen interaction, the draft genome assembly of isolate YZU573 from cucumber leaves with symptoms of PM was obtained with a hybrid approach, combining nanopore long-read and llumina paired-end sequencing. The final YZU573 genome assembly of 152.7 Mb consists of 58 contigs, with an N50 value of 0.75 Mb and 6491 predicted protein-coding genes. The effector analysis using the whole-genome sequence information revealed a total of 87 putative effector candidates, and 65 of them had their analogs, whereas the remaining 22 were novel ones. The new genome provides valuable resources to better understand plant-microbe interaction in cucumber PM disease.

摘要

是一种著名的专性活体营养型病原菌,可导致葫芦科植物发生白粉病(PM),是全球黄瓜生产最重要的限制因素之一。为了更好地了解该物种中已知参与宿主-病原体相互作用的无毒效应蛋白,采用纳米孔长读长测序和Illumina双端测序相结合的混合方法,获得了来自有PM症状的黄瓜叶片的分离株YZU573的基因组草图组装。YZU573的最终基因组组装大小为152.7 Mb,由58个重叠群组成,N50值为0.75 Mb,预测有6491个蛋白质编码基因。利用全基因组序列信息进行的效应子分析共鉴定出87个假定的效应子候选物,其中65个有类似物,其余22个是新的。新的基因组为更好地理解黄瓜白粉病中植物-微生物相互作用提供了宝贵资源。

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