Parusnath Myuri, Naidoo Yougasphree, Singh Moganavelli, Rihan Hail, Dewir Yaser Hassan
School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa.
School of Biological and Marine Sciences, Faculty of Science and Engineering, University of Plymouth, Drake Circus PL4 8AA, UK.
Plants (Basel). 2023 Apr 19;12(8):1702. doi: 10.3390/plants12081702.
The demand for medicinal plants is on a rise due to their affordability, accessibility and relatively non-toxic nature. (Combretaceae) is used in African traditional medicine to treat a number of diseases. This study aimed to screen the phytochemical composition of the hexane, chloroform and methanol extracts of . leaves and stems using qualitative phytochemical screening. Additionally, the study aimed to identify the functional phytochemical groups, determine the elemental composition and provide a fluorescence characterization of the powdered leaves and stems by performing Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray (EDX) microanalyses and fluorescence microscopy. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenolic compounds, polyphenols, terpenoids, tannins, coumarins, saponins, phytosterols, gums, mucilage, carbohydrates, amino acids and proteins within all leaf and stem extracts. Lipids and fixed oils were additionally present within the methanol extracts. FTIR demonstrated significant peaks in absorption frequency in the leaf at wavelengths of 3283.18, 2917.81, 1617.72, 1318.83, 1233.97, 1032.32 and 521.38 cm, and in the stem at 3318.91, 1619.25, 1317.13, 1032.68, 780.86 and 516.39 cm. These corresponded to the functional groups of chemical compounds including alcohols, phenols, primary amines, alkyl halides, alkanes and alkyl aryl ethers, corroborating the presence of the detected phytochemicals within the plant. EDX microanalyses showed the elemental composition of the powdered leaves (68.44% C, 26.72% O, 1.87% Ca, 0.96% Cl, 0.93% Mg, 0.71% K, 0.13% Na, 0.12 % Mn and 0.10% Rb) and stems (54.92% C, 42.86% O, 1.7% Ca, 0.43% Mg and 0.09% Mn). Fluorescence microscopy provided a characteristic evaluation of the plant in its powdered form and revealed distinct colour changes in the material when treated with various reagents and viewed under ultraviolet light. In conclusion, the phytochemical constituents of the leaves and stems of . confirm the suitability of this species for use in traditional medicine. The findings from this study suggest the need to validate the use of . in the development of modern medicines.
由于药用植物价格亲民、易于获取且相对无毒，对其需求正在上升。（使君子科）在非洲传统医学中用于治疗多种疾病。本研究旨在通过定性植物化学筛选来检测[植物名称]叶和茎的己烷、氯仿和甲醇提取物的植物化学成分。此外，该研究旨在识别功能性植物化学基团，确定元素组成，并通过傅里叶变换红外光谱（FTIR）、能量色散X射线（EDX）微分析和荧光显微镜对叶和茎粉末进行荧光表征。植物化学筛选显示，所有叶和茎提取物中均存在生物碱、黄酮类化合物、酚类化合物、多酚、萜类化合物、单宁、香豆素、皂苷、植物甾醇、树胶、黏液、碳水化合物、氨基酸和蛋白质。甲醇提取物中还存在脂质和固定油。FTIR显示，叶在波长为3283.18、2917.81、1617.72、1318.83、1233.97、1032.32和521.38 cm处有显著的吸收频率峰，茎在3318.91、1619.25、1317.13、1032.68、780.86和516.39 cm处有显著吸收频率峰。这些对应于包括醇、酚、伯胺、卤代烃、烷烃和烷基芳基醚在内的化合物的官能团，证实了植物中检测到的植物化学物质的存在。EDX微分析显示了叶粉末（68.44% C、26.72% O、1.87% Ca、0.96% Cl、0.93% Mg、0.71% K、0.13% Na、0.12% Mn和0.10% Rb）和茎（54.92% C、42.86% O、1.7% Ca、0.43% Mg和0.09% Mn）的元素组成。荧光显微镜对该植物粉末形式进行了特征评估，并显示当用各种试剂处理并在紫外光下观察时，材料有明显的颜色变化。总之，[植物名称]叶和茎的植物化学成分证实了该物种适用于传统医学。本研究结果表明有必要验证[植物名称]在现代药物开发中的应用。
