Liu Fangyan, Shen Chen, Chen Xuelian, Gao Fei, Chen Yin
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China.
Polymers (Basel). 2023 Apr 19;15(8):1933. doi: 10.3390/polym15081933.
The nanogels made from these polysaccharides and their derivatives are often used to construct drug delivery systems owing to their biocompatible, biodegradable, non-toxic, water-soluble, and bioactive characteristics. In this work, a novel pectin with unique gelling properties was extracted from the seed of (NPGP). The structural research indicated that NPGP was a low methoxyl pectin with a high content of galacturonic acid. NPGP-based nanogels (NGs) were accomplished employing the water in oil (W/O) nano-emulsion method. The cysteamine containing reduction-responsive bond and integrin-targeting RGD peptide were also grafted onto NPGP. The anti-tumor drug doxorubicin hydrochloride (DOX) was loaded during the formation of NGs, and the performance of DOX delivery was studied. The NGs were characterized by UV-vis, DLS, TEM, FT-IR, and XPS. The results showed that the prepared NGs were nanosized (167.6 ± 53.86 nm), had excellent encapsulation efficiency (91.61 ± 0.85%), and possessed a fine drug loading capacity (8.40 ± 0.16%). The drug release experiment showed that DOX@NPGP-SS-RGD had good redox-responsive performance. Furthermore, the results of cell experiments revealed good biocompatibility of prepared NGs, along with selective absorption by HCT-116 cells through integrin receptor-mediated endocytosis to play an anti-tumor effect. These studies indicated the potential application of NPGP-based NGs as targeted drug delivery systems.
由这些多糖及其衍生物制成的纳米凝胶，因其具有生物相容性、可生物降解、无毒、水溶性和生物活性等特性，常被用于构建药物递送系统。在本研究中，从[具体植物名称]种子中提取了一种具有独特胶凝特性的新型果胶（NPGP）。结构研究表明，NPGP是一种半乳糖醛酸含量高的低甲氧基果胶。采用油包水（W/O）纳米乳液法制备了基于NPGP的纳米凝胶（NGs）。还将含有还原响应键的半胱胺和整合素靶向RGD肽接枝到NPGP上。在NGs形成过程中负载抗肿瘤药物盐酸阿霉素（DOX），并研究了DOX的递送性能。通过紫外可见光谱、动态光散射、透射电子显微镜、傅里叶变换红外光谱和X射线光电子能谱对NGs进行了表征。结果表明，所制备的NGs为纳米尺寸（167.6±53.86 nm），具有优异的包封率（91.61±0.85%）和良好的载药能力（8.40±0.16%）。药物释放实验表明，DOX@NPGP-SS-RGD具有良好的氧化还原响应性能。此外，细胞实验结果显示所制备的NGs具有良好的生物相容性，并且HCT-116细胞通过整合素受体介导的内吞作用进行选择性吸收，从而发挥抗肿瘤作用。这些研究表明基于NPGP的NGs作为靶向药物递送系统具有潜在的应用价值。
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