Structural characterization and intestinal protection activity of polysaccharides from Sea buckthorn (Hippophae rhamnoides L.) berries.

The sea buckthorn (Hippophae rhamnoides L.) berries are rich in various bioactive components and widely used as fruit and traditional medicine. In this study, a novel heteropolysaccharide fraction (SP0.1-1) was isolated from Sea buckthorn berries. SP0.1-1 is composed of mannose, glucose, galactose, and arabinose in the molar ratio of 1:2.3:1.9:11.2 with a core structure containing 1,4-linked-α-d-Glcp, 1,4,6-linked-α-d-Glcp and 1,4-linked-α-d-Manp residues as the backbone. And the side-chains comprised of 1,3,5-linked-α-l-Araf, 1,5-linked-α-l-Araf, terminal α-Araf and 1,4-linked-β-d-Galp. Furthermore, a diet supplemented with SP0.1-1 extended the mean lifespan, enhanced antioxidant enzyme (superoxide dismutase, SOD; glutathione peroxidase, GSH-Px; and catalase, CAT) activities, and decreased the malondialdehyde (MDA) level and hydrogen peroxide (HO)-induced mortality rate in fruit flies (Drosophila melanogaster). To summarize, the study's findings will provide evidence for the development of sea buckthorn polysaccharide products.