Nelson H A, Lucas S V, Gibson T P
J Chromatogr. 1979 Jun 11;163(2):169-77. doi: 10.1016/s0378-4347(00)81460-7.
A method combining high-performance liquid chromatography for separation of digoxin from three of its metabolites with subsequent quantitation of each compound by radioimmunoassay is described. The metabolites are shown to interfere with the radioimmunoassay procedure thus providing the need for separation prior to assay.
本文描述了一种方法，该方法结合高效液相色谱法从地高辛的三种代谢物中分离出地高辛，随后通过放射免疫测定法对每种化合物进行定量。结果表明，这些代谢物会干扰放射免疫测定程序，因此需要在测定前进行分离。
