Anal Chem. 2010 Mar 1;82(5):1601-4. doi: 10.1021/ac9027615.
We report on the novel protocol for enzyme immobilization into gel of siloxanes using water-organic mixtures with the high content of organic solvent as a reaction medium. Hydrolysis of alkoxysilanes carried out without excessive dilution with water resulted in more active and stable enzyme containing membranes. Immobilization of an inherently labile lactate oxidase according to the proposed sol-gel protocol over Prussian Blue modified electrode resulted in an advanced lactate biosensor characterized with a sensitivity of 0.18 A M(-1) cm(-2) in the flow injection analysis (FIA) mode over a wide dynamic range. A comparison with the known sensors has shown that analytical performances of the elaborated lactate biosensor are advantageous over both published systems and commercialized devices. The biosensor shows an appropriate stability and is suitable for clinical analysis (including noninvasive diagnostics) and food quality control.
我们报告了一种新的酶固定化方法，即将硅氧烷凝胶中的酶固定在高有机溶剂含量的水-有机溶剂混合物中作为反应介质。在不进行过度水稀释的情况下，烷氧基硅烷的水解可以得到更具活性和稳定性的酶含膜。根据所提出的溶胶-凝胶方案，将固有不稳定的乳酸氧化酶固定在普鲁士蓝修饰电极上，得到了一种先进的乳酸生物传感器，在流动注射分析（FIA）模式下具有 0.18 A M(-1) cm(-2)的灵敏度，动态范围较宽。与已知传感器的比较表明，所研制的乳酸生物传感器的分析性能优于已发表的系统和商业化设备。该生物传感器具有适当的稳定性，适用于临床分析（包括非侵入性诊断）和食品质量控制。
