用于通过表面等离子体共振成像检测神经纤毛蛋白-1的新型生物传感器。
New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging.
机构信息
Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland.
Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, Poland.
出版信息
Sensors (Basel). 2023 Apr 19;23(8):4118. doi: 10.3390/s23084118.
Neuropilin-1 is transmembrane protein with soluble isoforms. It plays a pivotal role in both physiological and pathological processes. NRP-1 is involved in the immune response, formation of neuronal circuits, angiogenesis, survival and migration of cells. The specific SPRI biosensor for the determination of neuropilin-1 was constructed using mouse monoclonal antibody that captures unbound NRP-1 form body fluids. The biosensor exhibits linearity of the analytical signal between 0.01 and 2.5 ng/mL, average precision value 4.7% and recovery between 97% and 104%. The detection limit is 0.011 ng/mL, and the limit of quantification is 0.038 ng/mL. The biosensor was validated by parallel determination of NRP-1 in serum and saliva samples using the ELISA test, with good agreement of the results.
神经纤毛蛋白 1 是一种具有可溶性异构体的跨膜蛋白。它在生理和病理过程中都起着关键作用。NRP-1 参与免疫反应、神经元回路的形成、血管生成、细胞的存活和迁移。使用捕获体液中未结合的 NRP-1 形式的鼠单克隆抗体构建了用于测定神经纤毛蛋白 1 的特异性 SPRI 生物传感器。该生物传感器在 0.01 和 2.5ng/mL 之间表现出分析信号的线性，平均精密度值为 4.7%，回收率在 97%到 104%之间。检测限为 0.011ng/mL，定量限为 0.038ng/mL。使用 ELISA 试验平行测定血清和唾液样本中的 NRP-1，对生物传感器进行了验证，结果吻合良好。
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