Hızlı Fatih, Demirci Aykut, Benzer Emine, Hızlı Hatice, Başar Halil
Department of Urology, Dr. A.Y. Oncology Training and Research Hospital, University of Health Sciences, Demetevler, Ankara, Turkey.
Department of Pathology, Dr. A.Y. Oncology Training and Research Hospital, University of Health Sciences, Demetevler, Ankara, Turkey.
Int Urol Nephrol. 2023 Jun;55(6):1421-1426. doi: 10.1007/s11255-023-03613-1. Epub 2023 Apr 28.
To determine the effect of heparin administered during the early post urethral trauma period on inflammation and spongiofibrosis in rats.
The study included 24 male rats that were randomized into 3 groups of 8 each. The urethra was traumatized using a 24-G needle sheath in all rats. Group 1 (control group) received intraurethral saline 0.9% injected b.i.d. for 27 days, group 2 received intraurethral Na-heparin (liquemine-Roche) 1500 IU kg injected b.i.d. for 27 days, and group 3 received intraurethral Na-heparin 1500 IU kg injected b.i.d and saline 0.9% s.i.d. for 27 days. On day 28 the rats' penises were degloved and penectomy was performed. Inflammation, spongiofibrosis, and congestion in the urethra were investigated in each group.
A statistically significant difference was found between the three groups (control, heparin, and heparin + saline) in the histopathological status of spongiofibrosis, inflammation, and congestion, respectively (P = 0.0001, P = 0.002, P = 0.0001). Severe spongiofibrosis was observed in six (75%) of the rats in group 1 (control group), whereas severe spongiofibrosis was not observed in group 2 (heparin) or group 3 (heparin + saline).
We observed that intraurethral Na-heparin 1500 IU kg injectioned during the early posturethral trauma period in rats significantly decreased inflammation, spongiofibrosis, and congestion.
确定尿道创伤后早期给予肝素对大鼠炎症和海绵体纤维化的影响。
本研究纳入24只雄性大鼠，随机分为3组，每组8只。所有大鼠均使用24G针鞘造成尿道创伤。第1组（对照组）每天两次经尿道注射0.9%生理盐水，共27天；第2组每天两次经尿道注射1500 IU/kg钠肝素（速碧林 - 罗氏），共27天；第3组每天两次经尿道注射1500 IU/kg钠肝素，并每天一次经尿道注射0.9%生理盐水，共27天。在第28天，将大鼠阴茎去套并进行阴茎切除。对每组大鼠的尿道炎症、海绵体纤维化和充血情况进行研究。
三组（对照组、肝素组和肝素 + 生理盐水组）在海绵体纤维化、炎症和充血的组织病理学状态方面分别存在统计学显著差异（P = 0.0001，P = 0.002，P = 0.0001）。第1组（对照组）8只大鼠中有6只（75%）观察到严重海绵体纤维化，而第2组（肝素组）和第3组（肝素 + 生理盐水组）未观察到严重海绵体纤维化。
我们观察到大鼠尿道创伤后早期经尿道注射1500 IU/kg钠肝素可显著减轻炎症、海绵体纤维化和充血。
