Topping M D, Venables K M, Luczynska C M, Howe W, Taylor A J
J Allergy Clin Immunol. 1986 Jun;77(6):834-42. doi: 10.1016/0091-6749(86)90381-7.
Patients with work-related respiratory symptoms caused by inhaled acid anhydrides (trimellitic (TMA), phthalic (PA), tetrachlorophthalic (TCPA), and maleic anhydrides) have specific IgE antibody. The antibody is specific for a conjugate of the sensitizing anhydride (the hapten) and human serum albumin (HSA). We have investigated the specificity of the reaction to determine whether the antibody is directed against (1) the anhydride, (2) new antigenic determinants formed by conjugation of albumin with the anhydride, or (3) the complete anhydride-HSA conjugate. For the patients sensitized to TCPA and TMA, RAST inhibition studies demonstrate the anhydride-HSA conjugate to be a more effective inhibitor of RAST than the sodium salt of the anhydride or an anhydride-bovine serum albumin conjugate, whereas for those sensitized to PA, the free hapten is almost as an effective inhibitor as the conjugate. With each sera HSA conjugates of anhydrides to which the patient is not sensitized are weaker inhibitors than the sensitizing anhydride-albumin conjugate. These results provide strong evidence that for the patients sensitized to TCPA and TMA, the antibody combines with the anhydride and the spatially adjacent portion of the HSA molecule, whereas in the patients sensitized to PA, the antibody is specific for the hapten.
因吸入酸酐（偏苯三酸酐（TMA）、邻苯二甲酸酐（PA）、四氯邻苯二甲酸酐（TCPA）和顺丁烯二酸酐）而出现与工作相关呼吸道症状的患者具有特异性IgE抗体。该抗体对致敏酸酐（半抗原）与人血清白蛋白（HSA）的结合物具有特异性。我们已对该反应的特异性展开研究，以确定抗体是否针对（1）酸酐、（2）白蛋白与酸酐结合形成的新抗原决定簇，或（3）完整的酸酐 - HSA结合物。对于对TCPA和TMA致敏的患者，放射性变应原吸附试验（RAST）抑制研究表明，酸酐 - HSA结合物比酸酐的钠盐或酸酐 - 牛血清白蛋白结合物更能有效抑制RAST，而对于对PA致敏的患者，游离半抗原几乎与结合物一样是有效的抑制剂。对于每位患者，其未致敏的酸酐与HSA的结合物作为抑制剂的效果比致敏的酸酐 - 白蛋白结合物弱。这些结果提供了有力证据，即对于对TCPA和TMA致敏的患者，抗体与酸酐以及HSA分子在空间上相邻的部分结合，而对于对PA致敏的患者，抗体对半抗原有特异性。
