Greenlee J E
J Gen Virol. 1986 Jun;67 ( Pt 6):1109-14. doi: 10.1099/0022-1317-67-6-1109.
Nude (nu/nu) mice were inoculated intracranially with 10(6.5) newborn mouse 50% lethal doses of K virus and were studied over a period of 28 weeks using serological methods, virus assay and immunohistological staining for viral antigens. K virus infection of nude mice, although clinically asymptomatic, was slowly progressive despite prompt IgM and IgG antibody response. The highest titres of K virus infectivity were reached in spleens, kidneys and intestines. Vascular endothelial cells represented the major site of viral replication, as has been shown to be the case in immunologically normal mice, with extensive involvement of intestinal capillaries. In addition, however, unlike immunologically normal mice, nude mice inoculated with K virus developed multifocal infection of renal tubular epithelial cells. Nude mice did not develop histologically detectable evidence of central nervous system involvement by K virus, and K virus infection did not result in neoplasia. Infected vascular endothelial cells and renal tubular epithelial cells in animals studied at 16 and 27 weeks after inoculation were grouped in scattered clusters, suggesting local spread of infection. The present study indicates that nude mice with preserved B cell function but impaired T cell-mediated immunity are able to limit systemic dissemination of K virus but are unable to prevent local progression of infection by cell-to-cell spread. K virus is capable of altering its cellular tropism during chronic infection.
将10（6.5）个新生小鼠50%致死剂量的K病毒颅内接种到无胸腺（nu/nu）小鼠体内，并使用血清学方法、病毒检测和病毒抗原免疫组织化学染色对其进行了28周的研究。无胸腺小鼠感染K病毒后，尽管临床上无症状，但尽管迅速出现IgM和IgG抗体反应，感染仍呈缓慢进展。脾脏、肾脏和肠道中的K病毒感染性滴度最高。血管内皮细胞是病毒复制的主要部位，这与免疫正常小鼠的情况相同，肠道毛细血管广泛受累。然而，除此之外，与免疫正常小鼠不同的是，接种K病毒的无胸腺小鼠出现了肾小管上皮细胞的多灶性感染。无胸腺小鼠未出现组织学上可检测到的K病毒累及中枢神经系统的证据，且K病毒感染未导致肿瘤形成。在接种后16周和27周研究的动物中，受感染的血管内皮细胞和肾小管上皮细胞呈散在簇状分布，提示感染的局部扩散。本研究表明，B细胞功能保留但T细胞介导的免疫受损的无胸腺小鼠能够限制K病毒的全身扩散，但无法阻止通过细胞间传播的感染局部进展。K病毒在慢性感染期间能够改变其细胞嗜性。
