Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 573 Tulip Street, Changchun, 130122, Jilin, China; College of Veterinary Medicine, Jilin University, 5333 Xi'an Avenue, Changchun, 130062, Jilin, China.
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 573 Tulip Street, Changchun, 130122, Jilin, China; College of Veterinary Medicine, Hebei Agricultural University, 2596 Lucky South Street, Baoding, 071000, Hebei, China.
Int J Hyg Environ Health. 2023 Jun;251:114173. doi: 10.1016/j.ijheh.2023.114173. Epub 2023 Apr 27.
Poultry farms are a complex environment for close contact between humans and animals. Accumulating evidence has indicated that pathogens and drug resistance genes in chicken houses may pose a serious threat to public health and economic concerns. However, insufficient knowledge of the indoor aerosol microbiome and resistome profiles of layer hen houses hampers the understanding of their health effects. Environmental surveillance of antibiotic resistance may contribute to a better understanding and management of the human exposure risk of bioaerosols under the environmental conditions of chicken houses. In addition, the chicken house has a long operation cycle, and the bacterial diversity and antibiotic resistance genes of aerosols in different periods may be different. In this study, air samples were collected from 18 chicken houses on three farms, including the early laying period (EL), peak laying period (PL), and late laying period (LL). 16S rRNA gene sequencing and metagenomics were used to study the composition of the bacteria and resistome in aerosols of layer hen houses and the results showed that they varied with laying period. The highest alpha diversity of bacteria was observed in PL bioaerosols. The dominant bacterial phyla included Firmicutes, Bacteroidetes and Proteobacteria. Three potential pathogenic bacterial genera (Bacteroides, Corynebacterium and Fusobacterium) were found. The most abundant ARG type was aminoglycosides in all laying periods. In total, 22 possible ARG host genera were detected. ARG subtypes and abundance were both higher in LL. Network analysis also showed higher co-occurrence patterns between the bacteria and resistome in bioaerosols. The laying period plays an important role in the bacterial community and resistome in layer house aerosols.
家禽养殖场是人类与动物密切接触的复杂环境。越来越多的证据表明，鸡舍中的病原体和耐药基因可能对公共卫生和经济问题构成严重威胁。然而，由于对鸡舍室内气溶胶微生物组和耐药组谱的了解不足，阻碍了对其健康影响的理解。对抗生素耐药性的环境监测有助于更好地了解和管理在鸡舍环境条件下生物气溶胶对人类暴露风险。此外，鸡舍的运行周期较长，不同时期气溶胶中的细菌多样性和抗生素耐药基因可能不同。在这项研究中，从三个农场的 18 个鸡舍中采集了空气样本，包括早期产蛋期 (EL)、高峰期产蛋期 (PL) 和后期产蛋期 (LL)。使用 16S rRNA 基因测序和宏基因组学研究了蛋鸡舍气溶胶中细菌和耐药组的组成，结果表明它们随产蛋期而变化。PL 生物气溶胶中观察到细菌的最高 alpha 多样性。优势细菌门包括厚壁菌门、拟杆菌门和变形菌门。发现了三个潜在的致病性细菌属 (拟杆菌属、棒状杆菌属和梭菌属)。所有产蛋期丰度最高的 ARG 类型是氨基糖苷类。总共检测到 22 种可能的 ARG 宿主属。LL 中 ARG 亚型和丰度均较高。网络分析还显示了生物气溶胶中细菌和耐药组之间更高的共同发生模式。产蛋期在蛋鸡舍气溶胶中的细菌群落和耐药组中起着重要作用。
