木薯（Manihot esculenta Crantz）地方品种采后生理劣变（PPD）评估的代谢指纹图谱。

Metabolite fingerprinting of cassava (Manihot esculenta Crantz) landraces assessed for post-harvest physiological deterioration (PPD).

机构信息

CIRAD-VARTC, P.O. Box 946, Port-Vila, Vanuatu; UMR AGAP Institut, Université de Montpellier, CIRAD, INRAE, Institut Agro, F-34398 Montpellier, France.

VARTC, P.O. Box 231, Luganville, Vanuatu.

出版信息

Food Chem. 2023 Sep 30;421:136217. doi: 10.1016/j.foodchem.2023.136217. Epub 2023 Apr 18.

DOI:10.1016/j.foodchem.2023.136217

PMID:37121018

Abstract

Cassava landraces are impacted by post-harvest physiological deterioration (PPD). 34 primary/secondary metabolites (carotenes, flavonols, indols, phenolic, hydroxycinnamic, and organic acids) were analysed using HPLC/GC-MS in 72 landraces harvested 8 months after planting (MAP) to clarify whether these compounds may play a role in PPD tolerance. Cluster analysis differentiated a first group with high organic acids contents, citric acid being dominant, a second group with landraces high in tryptophan, a third group including landraces with high phenolic and hydroxycinnamic acids content, and a fourth group characterised by 8 carotenoids. PPD tolerant and susceptible landraces were present in each group. To determine if PPD is related to age of harvest, 174 landraces were harvested at 6, 8, 10 and 12 MAP. Scopoletin, sucrose and glucose were analysed. PPD was positively correlated with DMC and negatively correlated with scopoletin at all ages of harvest. Scopoletin is a useful biomarker to characterize landraces.

摘要

木薯地方品种受收获后生理劣变（PPD）的影响。采用 HPLC/GC-MS 分析了 72 个种植 8 个月后收获的地方品种中的 34 种主要/次要代谢物（类胡萝卜素、类黄酮、吲哚、酚类、羟基肉桂酸和有机酸），以阐明这些化合物是否可能在 PPD 耐受中发挥作用。聚类分析将具有高有机酸含量的第一个组与柠檬酸为主的组、色氨酸含量高的第二个组、酚类和羟基肉桂酸含量高的第三个组以及以 8 种类胡萝卜素为特征的第四个组区分开来。每个组中都存在 PPD 耐受和易感的地方品种。为了确定 PPD 是否与收获年龄有关，对 174 个地方品种在 6、8、10 和 12 MAP 时进行了收获。分析了东莨菪内酯、蔗糖和葡萄糖。在所有收获年龄，PPD 与 DMC 呈正相关，与东莨菪内酯呈负相关。东莨菪内酯是一种有用的生物标志物，可以用来表征地方品种。