Tsuji Y, Yamada K, Hosoya N, Moriuchi S
J Nutr Sci Vitaminol (Tokyo). 1986 Feb;32(1):93-100. doi: 10.3177/jnsv.32.93.
The bioavailability of newly developed sugar substitutes was observed by measuring the transmural potential difference (delta PD) evoked by Na+-dependent active transport of glucose, which is supposed to be produced by the hydrolysis of sugar substitutes. delta PD was measured using everted intestinal sac prepared from jejunum of adult rats and compared with the digestibility of sugar substitutes in the mucosal homogenate of everted sac. delta PDs evoked by glucose, maltose or maltosylfructose had almost the same levels, however, the delta PD evoked by sucrose was a little lower. delta PDs evoked by maltitol or palatinose were low, and delta PDs evoked by fructo-oligosaccharides were negligible. The hydrolyzing activities of these sugars and sugar substitutes by the mucosal homogenate were correlated with the delta PDs. A significant positive correlation was observed between delta PDmax of various sugars and sugar substitutes and the Vmax of their corresponding hydrolyzing activities. Also, a significant positive correlation was observed between Kt and Km values of these sugars. These results suggest that the absorption of sugar substitutes is dependent on digestibility by membrane digestive enzymes.
通过测量由葡萄糖的钠依赖性主动转运所诱发的跨膜电位差（δPD）来观察新开发的糖替代品的生物利用度，这种主动转运被认为是由糖替代品的水解产生的。使用成年大鼠空肠制备的外翻肠囊测量δPD，并将其与糖替代品在外翻囊黏膜匀浆中的消化率进行比较。由葡萄糖、麦芽糖或麦芽低聚糖诱发的δPD水平几乎相同，然而，由蔗糖诱发的δPD略低。由麦芽糖醇或帕拉金糖诱发的δPD较低，由低聚果糖诱发的δPD可忽略不计。这些糖类和糖替代品被黏膜匀浆的水解活性与δPD相关。观察到各种糖类和糖替代品的最大δPD与其相应水解活性的Vmax之间存在显著正相关。此外，还观察到这些糖类的Kt和Km值之间存在显著正相关。这些结果表明，糖替代品的吸收取决于膜消化酶的消化率。
