Bestetti Reinaldo B, Dellalibera-Joviliano Renata, Rizzi Ellen, Bonacio Giselle F, Faria-Jr Milton, Furlan-Daniel Rosemeire, Castro-França Suzeley
Department of Medicine, UNAERP Medical School, University of Ribeirão Preto, Ribeirão Preto, Brazil.
Department of Biotechnology, University of Ribeirão Preto, Ribeirão Preto, Brazil.
Cardiol Res Pract. 2023 Apr 19;2023:8484697. doi: 10.1155/2023/8484697. eCollection 2023.
Chronic Chagas heart disease (CCHD) and systemic arterial hypertension (SAH) frequently coexists in areas where Chagas disease is endemic. The effects of the association of both conditions (CCHD-SAH) on the extracellular matrix (ECM) remodeling are unknown. Matrix metalloproteinases (MMP) 2 and 9 are involved in ECM remodeling. The aim of this study was to evaluate MMP 2 and MMP9 in CCHD-SAH patients and to correlate their levels with those of the profibrogenic cytokine TGF-beta.
We included 19 patients with CCHD-SAH, 14 patients with CCHD alone, and 19 controls matched by sex and age. MMP-2 and MMP-9 plasma levels were studied by gel zymography and showed as optical densities (OD). TGF-beta plasma levels were measured by double-ligand ELISA and expressed as pg/mL.
Median (5, 95) MMP-2 plasma levels were 1224.7 OD (1160, 1433.5) in patients with CCHD alone, 1424.1 OD (1267.5, 1561) in patients with CCHD-SAH, and 940 OD (898.1, 1000.8) in controls (=0.001). MMP-9 plasma levels were 1870 OD (1740, 1904.1) in patients with CCHD alone, 1754.6 OD (1650, 2049) in those with CCHD-SAH and 89.7 OD (80, 96) in controls (=0.0003). MMP-9 plasma levels were higher than those of MMP 2 in patients with CCHD-SAH (=0.01). No correlation was found between TGF-beta plasma levels with MMP-2 serum levels ( = 0.12; =0.7), but a moderate negative correlation ( = -0.46; =0.048) was observed between TGF-beta and MMP-9 plasma levels.
MMP-2 and especially MMP-9 may play a role in the ECM remodeling process in patients with CCHD-SAH. TGF-Beta may counteract the MMP effect on the ECM remodeling process in patients with CCHD-SAH.
在恰加斯病流行地区，慢性恰加斯心脏病（CCHD）和系统性动脉高血压（SAH）常常并存。两种疾病（CCHD-SAH）联合对细胞外基质（ECM）重塑的影响尚不清楚。基质金属蛋白酶（MMP）2和9参与ECM重塑。本研究的目的是评估CCHD-SAH患者的MMP 2和MMP9，并将其水平与促纤维化细胞因子转化生长因子-β（TGF-β）的水平相关联。
我们纳入了19例CCHD-SAH患者、14例单纯CCHD患者以及19例年龄和性别匹配的对照组。通过凝胶酶谱法研究MMP-2和MMP-9的血浆水平，并以光密度（OD）表示。通过双配体ELISA法测量TGF-β的血浆水平，并以pg/mL表示。
单纯CCHD患者的MMP-2血浆水平中位数（第5、95百分位数）为1224.7 OD（1160，1433.5），CCHD-SAH患者为1424.1 OD（1267.5，1561），对照组为940 OD（898.1，1000.8）（P=0.001）。单纯CCHD患者的MMP-9血浆水平为1870 OD（1740，1904.1），CCHD-SAH患者为1754.6 OD（1650，2049），对照组为89.7 OD（80，96）（P=0.0003）。CCHD-SAH患者的MMP-9血浆水平高于MMP 2（P=0.01）。未发现TGF-β血浆水平与MMP-2血清水平之间存在相关性（r=0.12；P=0.7），但观察到TGF-β与MMP-9血浆水平之间存在中度负相关（r=-0.46；P=0.048）。
MMP-2尤其是MMP-9可能在CCHD-SAH患者的ECM重塑过程中起作用。TGF-β可能抵消MMP对CCHD-SAH患者ECM重塑过程的影响。
