Guo Yinhua, Tong Xingli, Tang Peipei, Zuo Wenting, Tan Yong
Department of Reproductive Medicine, Jiangsu Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210000, Jiangsu, China.
Evid Based Complement Alternat Med. 2023 Apr 20;2023:9402155. doi: 10.1155/2023/9402155. eCollection 2023.
Currently, exploring effective agents is urgently required for polycystic ovary syndrome (PCOS) treatment. Although nourishing kidney promoting ovulation decoction (NKPOD) as a traditional Chinese medicine decoction is widely employed to increase pregnancy rates, whether NKPOD attenuates ovulation disorders in PCOS patients remains unknown. Here, we aim to explore the clinical significance and the underlying mechanisms of NKPOD in ovulation disorders.
PCOS patients were recruited to confirm the clinical significance of NKPOD in attenuating ovulation disorder. Subsequently, regulation targets of NKPOD were identified through network pharmacology analysis. Additionally, a series of experiments were performed to observe the impacts of NKPOD on miRNA-224 transcription through transcription factor AR.
In this study, NKPOD administration improved hormone dysregulation and reproductive outcomes in PCOS patients. Interestingly, 100 potential targets related to NKPOD and PCOS were screened, and transcription regulation was observed to be the most enriched function. Mechanistically, NKPOD inhibited miRNA-224 transcription through reducing AR expression, in which AR as a transcription factor directly regulated miRNA-224 transcription.
Collectively, these findings highlight the therapeutic effect of NKPOD on PCOS, which could provide promising therapeutic agents for PCOS.
目前，多囊卵巢综合征（PCOS）治疗急需探索有效的药物。尽管补肾促排卵汤（NKPOD）作为一种中药汤剂被广泛用于提高妊娠率，但NKPOD是否能减轻PCOS患者的排卵障碍仍不清楚。在此，我们旨在探讨NKPOD在排卵障碍中的临床意义及潜在机制。
招募PCOS患者以证实NKPOD在减轻排卵障碍方面的临床意义。随后，通过网络药理学分析确定NKPOD的调控靶点。此外，进行了一系列实验以观察NKPOD通过转录因子AR对miRNA-224转录的影响。
在本研究中，给予NKPOD改善了PCOS患者的激素失调和生殖结局。有趣的是，筛选出了100个与NKPOD和PCOS相关的潜在靶点，且观察到转录调控是最富集的功能。机制上，NKPOD通过降低AR表达抑制miRNA-224转录，其中AR作为转录因子直接调控miRNA-224转录。
总体而言，这些发现突出了NKPOD对PCOS的治疗作用，可为PCOS提供有前景的治疗药物。
