Vinodhini J, Rajendran L, Karthikeyan G
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003 India.
3 Biotech. 2023 May;13(5):143. doi: 10.1007/s13205-023-03576-1. Epub 2023 Apr 27.
Cucumber mosaic virus (CMV) is the one of notorious virus known for its ubiquitous nature and causes substantial yield loss worldwide. The resistance against the Cucumber mosaic virus (CMV) was envisaged in transgenic lines by introducing viral gene fragments The chimeric hairpin RNA constructs incorporating 401 bp of coat protein, 411 bp of replicase protein and 361 bp of 2b gene were developed respectively and transformed into . The regenerated transgenic lines introduced with inverted repeats of CMV gene fragments exhibited enhanced resistance against CMV. The preliminary molecular screening and qPCR confirmed the integration of transgene in the transgenic lines. The spectrum of resistance in transgenic lines was evaluated by challenge inoculation with CMV and the resistance was determined through DAC-ELISA. The complete resistance was achieved in the hpRNA-CP transformant with a very low titre (0.029) of CMV followed by hpRNA-REP (0.099) with no symptoms.
The online version contains supplementary material available at 10.1007/s13205-023-03576-1.
黄瓜花叶病毒（CMV）是一种臭名昭著的病毒，因其广泛存在的特性而闻名，在全球范围内导致大量产量损失。通过引入病毒基因片段，在转基因品系中设想了对黄瓜花叶病毒（CMV）的抗性。分别构建了包含401bp外壳蛋白、411bp复制酶蛋白和361bp 2b基因的嵌合发夹RNA构建体，并将其转化到……中。导入CMV基因片段反向重复序列的再生转基因品系对CMV表现出增强的抗性。初步分子筛选和qPCR证实了转基因在转基因品系中的整合。通过用CMV进行挑战接种来评估转基因品系的抗性谱，并通过双抗夹心ELISA（DAC-ELISA）测定抗性。在hpRNA-CP转化体中实现了完全抗性，CMV滴度非常低（0.029），其次是hpRNA-REP（0.099），没有症状。
在线版本包含可在10.1007/s13205-023-03576-1获取的补充材料。
