Xu Yunfeng, Guo Weiping, Luo Denglin, Li Peiyan, Xiang Jinle, Chen Junliang, Xia Xiaodong, Xie Qinggang
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, China.
School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, China.
Front Microbiol. 2023 Apr 14;14:1175912. doi: 10.3389/fmicb.2023.1175912. eCollection 2023.
is a common foodborne pathogen which can form biofilms to help them resist to antimicrobials. It brings great harm to human health. Punicalagin has good antimicrobial activities against , but its effect on biofilm formation has not been clearly illustrated. The aim of this study was to explore the antibiofilm effects of punicalagin against . Results showed that punicalagin did not significantly interfere with the growth of at the concentrations of 1/64 MIC to 1/16 MIC. The biomass and metabolic activity of biofilms were significantly reduced when exposed to sub-inhibitory concentrations of punicalagin. The number of viable cells in the biofilms was also decreased after punicalagin treatment. Scanning electron microscopy and confocal laser scanning microscopy images confirmed that punicalagin damaged the structure of biofilms. The antibiofilm mechanism was partly due to the modification of the cell surface which led to the reduction of cell surface hydrophobicity. These findings suggest that punicalagin has the potential to be developed as an alternative to control biofilms.
是一种常见的食源性病原体，它可以形成生物膜来帮助自身抵抗抗菌药物。它对人类健康造成极大危害。石榴皮鞣质对具有良好的抗菌活性，但其对生物膜形成的影响尚未得到明确阐明。本研究的目的是探讨石榴皮鞣质对的抗生物膜作用。结果表明，在1/64 MIC至1/16 MIC浓度下，石榴皮鞣质对的生长没有显著干扰。当暴露于亚抑制浓度的石榴皮鞣质时，生物膜的生物量和代谢活性显著降低。石榴皮鞣质处理后，生物膜中活细胞的数量也减少了。扫描电子显微镜和共聚焦激光扫描显微镜图像证实，石榴皮鞣质破坏了生物膜的结构。抗生物膜机制部分是由于细胞表面的改变导致细胞表面疏水性降低。这些发现表明，石榴皮鞣质有潜力被开发为控制生物膜的替代品。
