Wen Qing-Hui, Wang Rui, Zhao Si-Qi, Chen Bo-Ru, Zeng Xin-An
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510641, China.
Foods. 2021 Oct 28;10(11):2614. doi: 10.3390/foods10112614.
Taking into consideration the importance of biofilms in food deterioration and the potential risks of antiseptic compounds, antimicrobial agents that naturally occurring are a more acceptable choice for preventing biofilm formation and in attempts to improve antibacterial effects and efficacy. Citrus flavonoids possess a variety of biological activities, including antimicrobial properties. Therefore, the anti-biofilm formation properties of the citrus flavonoid naringenin on the ATCC 6538 () were investigated using subminimum inhibitory concentrations (sub-MICs) of 5~60 mg/L. The results were confirmed using laser and scanning electron microscopy techniques, which revealed that the thick coating of biofilms became thinner and finally separated into individual colonies when exposed to naringenin. The decreased biofilm formation of cells may be due to a decrease in cell surface hydrophobicity and exopolysaccharide production, which is involved in the adherence or maturation of biofilms. Moreover, transcriptional results show that there was a downregulation in the expression of biofilm-related genes and alternative sigma factor induced by naringenin. This work provides insight into the anti-biofilm mechanism of naringenin in and suggests the possibility of naringenin being used in the industrial food industry for the prevention of biofilm formation.
考虑到生物膜在食品变质中的重要性以及抗菌化合物的潜在风险，天然存在的抗菌剂是预防生物膜形成以及提高抗菌效果和功效的更可接受的选择。柑橘类黄酮具有多种生物活性，包括抗菌特性。因此，使用5至60 mg/L的亚最小抑菌浓度（亚MICs）研究了柑橘类黄酮柚皮苷对ATCC 6538（）的抗生物膜形成特性。使用激光和扫描电子显微镜技术证实了结果，结果表明，当暴露于柚皮苷时，生物膜的厚涂层变薄，最终分离成单个菌落。细胞生物膜形成的减少可能是由于细胞表面疏水性和胞外多糖产生的减少，这与生物膜的粘附或成熟有关。此外，转录结果表明，柚皮苷诱导生物膜相关基因和替代sigma因子的表达下调。这项工作深入了解了柚皮苷在中的抗生物膜机制，并表明柚皮苷有可能用于工业食品工业中预防生物膜形成。
