Neussel H
Zentralbl Bakteriol Orig A. 1978;242(3):375-86.
On the basis of a continuous culture method using the Biostat apparatus (Braun Melsungen) during a six hours interval the kinetics of growth inhibition and killing of E. coli were studied under the influence of varying concentrations of carbenicillin and cephalotin. The bacteria were incubated with the antibiotic at 37 degrees C, either in the broth of the culture vessel or incorporated into the depth of the agar of a dip slide which was brought into the uninoculated broth. Constant inhibitory concentrations (1 X MIC-4 X MIC) caused a bactericidal effect with both antibiotics. The recovery of the bacteria from the toxic effects of carbenicillin and cephalotin was slow if the concentrations were decreased to subinhibitory concentrations after initial contact with inhibitory peak levels. Scanning electron microscope studies demonstrated with both antibiotics a rapid filament formation with partial lysis.
在使用Biostat仪器（布劳恩·梅尔松根公司）的连续培养方法基础上，在6小时的时间间隔内，研究了不同浓度的羧苄青霉素和头孢噻吩对大肠杆菌生长抑制和杀灭的动力学。细菌在37℃下与抗生素一起培养，要么在培养容器的肉汤中，要么掺入浸片琼脂的深层，浸片再放入未接种的肉汤中。恒定的抑制浓度（1×最低抑菌浓度-4×最低抑菌浓度）对两种抗生素均产生杀菌作用。如果在最初接触抑制峰值水平后将浓度降至亚抑制浓度，细菌从羧苄青霉素和头孢噻吩的毒性作用中恢复缓慢。扫描电子显微镜研究表明，两种抗生素都会迅速形成细丝并伴有部分裂解。
