Kim Yeon-Ji, Kim Tae In, Kim Kyungho
Korean Medicine-Application Center, Korea Institute of Oriental Medicine, Daegu, Republic of Korea.
J Tradit Complement Med. 2023 Feb 21;13(3):285-296. doi: 10.1016/j.jtcme.2023.02.009. eCollection 2023 May.
Platelet-derived thrombosis is important in the pathogenesis of cardiovascular diseases. HTB is an optimized herbal medicine including Georgi, Juzepzuk, and Koidzumi. It is widely used in traditional medicine due to its anti-inflammatory and antioxidant effects. However, its antiplatelet and antithrombotic activities have not been completely validated. The current study aimed to examine the inhibitory effect of the novel herb formula HTB against platelet activation and thrombus formation.
The antiplatelet activities of HTB via platelet aggregation, granule secretion, reactive oxygen species generation, and intracellular calcium mobilization were evaluated. Moreover, the antithrombotic effect of HTB via FeCl-induced arterial thrombus formation in mice was assessed. The inhibitory effect of HTB against primary hemostasis was investigated based on transection tail bleeding time.
HTB treatment significantly inhibited glycoprotein VI-mediated platelet aggregation, granule secretion, reactive oxygen species generation, and intracellular calcium mobilization. Biochemical studies revealed that HTB inhibited glycoprotein VI-mediated platelet signal transduction during cell activation. Further, its antioxidant effect might be derived by reducing the phosphorylation of the p47/Hic5 axis signalosome. Oral HTB treatment was effective in decreasing FeCl-induced arterial thrombus formation without prolonging the tail bleeding time. HTB can be an effective therapeutic agent against thrombotic diseases.
血小板源性血栓形成在心血管疾病的发病机制中具有重要作用。HTB是一种优化的草药配方，包含地锦草、白背叶根和火炭母。因其抗炎和抗氧化作用，它在传统医学中被广泛应用。然而，其抗血小板和抗血栓活性尚未得到充分验证。本研究旨在考察新型草药配方HTB对血小板活化和血栓形成的抑制作用。
通过血小板聚集、颗粒分泌、活性氧生成和细胞内钙动员来评估HTB的抗血小板活性。此外，评估了HTB通过FeCl诱导小鼠动脉血栓形成的抗血栓作用。基于断尾出血时间研究了HTB对初级止血的抑制作用。
HTB处理显著抑制糖蛋白VI介导的血小板聚集、颗粒分泌、活性氧生成和细胞内钙动员。生化研究表明，HTB在细胞活化过程中抑制糖蛋白VI介导的血小板信号转导。此外，其抗氧化作用可能是通过减少p47/Hic5轴信号体的磷酸化来实现的。口服HTB治疗可有效减少FeCl诱导的动脉血栓形成，且不延长尾部出血时间。HTB可能是一种治疗血栓性疾病的有效药物。
