Digestive Disease Center, CHA University Bundang Medical Center, Seongnam, Korea.
CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seongnam, Korea.
Gut Liver. 2017 Sep 15;11(5):655-666. doi: 10.5009/gnl16496.
BACKGROUND/AIMS: In inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of (AM) or (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration.
Murine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge. Acute colitis was induced with 7 days of dextran sulfate sodium (DSS) in male C57BL/6 mice, and extracts of AM and TH were administered for 2 weeks before DSS administration.
studies demonstrated that AM or TH treatment reduced LPS-induced -2 and -α mRNA levels but increased heme oxygenase-1 (HO-1). Oral preadministration of AM and TH rescued mice from DSS-induced colitis by inhibiting inflammatory mediators via inactivated extracellular signal regulated kinase and repressed nuclear factor κB and signal transducer and activator of transcription 3, but the effect was weaker for sulfasalazine than that for the extracts. Anti-inflammatory activities occurred via the inhibition of macrophage and T lymphocyte infiltrations. Unlike sulfasalazine, which did not induce HO-1, TH extracts afforded significant HO-1 induction.
Because the AM or TH extracts were far superior in preventing DSS-induced colitis than sulfasalazine, AM or TH extracts can be considered natural agents that can prevent IBD relapse.
背景/目的：在炎症性肠病（IBD）中，患者会反复发作缓解和复发，并可能面临结肠炎相关癌症的风险。我们假设 （AM）或 （TH）的植物提取物可能比柳氮磺胺吡啶更适合治疗这种疾病，因为这些提取物可以促进额外的再生。
在脂多糖（LPS）诱导的挑战之前，用 AM 或 TH 预处理鼠肠上皮 IEC-6 细胞。雄性 C57BL/6 小鼠用葡聚糖硫酸钠（DSS）诱导急性结肠炎，在给予 DSS 之前给予 AM 和 TH 提取物 2 周。
研究表明，AM 或 TH 治疗可降低 LPS 诱导的 -2 和 -α mRNA 水平，但增加血红素加氧酶-1（HO-1）。AM 和 TH 的口服预先给药通过抑制激活的细胞外信号调节激酶和抑制核因子 κB 和信号转导和转录激活因子 3 来挽救 DSS 诱导的结肠炎小鼠，从而抑制炎症介质，但效果比提取物弱。抗炎活性通过抑制巨噬细胞和 T 淋巴细胞浸润发生。与不诱导 HO-1 的柳氮磺胺吡啶不同，TH 提取物可显著诱导 HO-1。
由于 AM 或 TH 提取物在预防 DSS 诱导的结肠炎方面远优于柳氮磺胺吡啶，因此 AM 或 TH 提取物可以被认为是预防 IBD 复发的天然药物。
