L-甲硫氨酸增强大鼠子宫平滑肌对乙酰胆碱和高钾氯化钾的收缩反应。

L-methionine enhances the contractile responses of rat uterine smooth muscle to acetylcholine and high KCl.

作者信息

Ichida S

出版信息

Jpn J Pharmacol. 1986 Mar;40(3):469-71. doi: 10.1254/jjp.40.469.

DOI:10.1254/jjp.40.469

Abstract

The contractile responses of isolated uterine smooth muscle from estradiol-treated ovariectomized rats to acetylcholine and high KCl in Ca-depleted modified Locke-Ringer (without CaCl2 and with 0.1 mM EGTA) solution on addition of CaCl2 (final concentration, 5 mM) are used as an indicator of Ca influx through the Ca channel. L-Methionine significantly enhanced these responses in the absence and presence of 3-deazaadenosine plus homocysteine thiolactone, blockers of methylation. These findings suggest that methylations of some components in isolated uterine smooth muscle seem to be important in stimulation-contraction coupling in the muscle.

摘要

将来自经雌二醇处理的去卵巢大鼠的离体子宫平滑肌在无钙的改良洛克林格液（不含氯化钙，含0.1 mM乙二醇双四乙酸）中对乙酰胆碱和高钾氯化钾的收缩反应，在添加氯化钙（终浓度5 mM）时作为通过钙通道的钙内流指标。L-甲硫氨酸在存在和不存在甲基化阻断剂3-脱氮腺苷加同型半胱氨酸硫内酯的情况下均显著增强了这些反应。这些发现表明，离体子宫平滑肌中某些成分的甲基化在肌肉的刺激-收缩偶联中似乎很重要。