Animal Facility, Institute of Cellular Physiology, National Autonomous University of Mexico, Mexico City, Mexico.
Department of Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of Mexico, Juriquilla, Quer??taro, Mexico.
J Am Assoc Lab Anim Sci. 2023 May 1;62(3):267-273. doi: 10.30802/AALAS-JAALAS-22-000081. Epub 2023 May 2.
Since 2015, the National Institutes of Health has called for its funded preclinical research to include both male and female subjects. However, much of the basic animal research that has studied heart rate and blood pressure in the past has used male rats. Male rats have been preferred for these studies to avoid the possible complicating effects of the female estrous cycle. The aim of the current study was to determine whether blood pressure and heart rates vary as a function of the estrous cycle phase of young normotensive Wistar-Kyoto (WKY) and Spontaneously Hypertensive (SHR) female rats. Blood pressure and heart rate were measured at the same time of day throughout the estrous cycle by using a noninvasive tail cuff sphygmomano- metric technique. As expected, 16-wk-old female SHR rats had higher blood pressure and heart rates than did age-matched female WKY rats. However, no significant differences in mean, systolic, or diastolic arterial blood pressure or heart rate were detected across the different stages of the estrous cycle in either strain of female rats. Consistent with previous reports, heart rates were higher and showed less variation in the hypertensive SHR female rats as compared with the normotensive WKY female rats. These results indicate that studies measuring blood pressure and heart rate can include young female SHR and WKY rats with no effect of estrous cycle stage.
自 2015 年以来，美国国立卫生研究院呼吁将其资助的临床前研究纳入男性和女性受试者。然而，过去许多研究心率和血压的基础动物研究都使用雄性大鼠。之所以选择雄性大鼠进行这些研究，是为了避免雌性发情周期可能带来的复杂影响。本研究旨在确定年轻正常血压的 Wistar-Kyoto（WKY）和自发性高血压（SHR）雌性大鼠的发情周期阶段是否会影响血压和心率。通过使用非侵入性尾套测压法，在发情周期的每天同一时间测量血压和心率。正如预期的那样，16 周龄的雌性 SHR 大鼠的血压和心率高于同龄的雌性 WKY 大鼠。然而，在两种雌性大鼠的发情周期的不同阶段，平均动脉血压、收缩压或舒张压或心率均无显著差异。与之前的报告一致，与正常血压的 WKY 雌性大鼠相比，高血压的 SHR 雌性大鼠的心率更高，变化更小。这些结果表明，测量血压和心率的研究可以包括年轻的雌性 SHR 和 WKY 大鼠，而不受发情周期阶段的影响。
